18-BTT EAGER: Controlling meiotic recombination in crops by manipulating DNA methylation

18-BTT EAGER:通过操纵 DNA 甲基化控制作物减数分裂重组

基本信息

  • 批准号:
    BB/S020918/1
  • 负责人:
  • 金额:
    $ 25.79万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2019
  • 资助国家:
    英国
  • 起止时间:
    2019 至 无数据
  • 项目状态:
    已结题

项目摘要

This consortium plans to develop highly innovative strategies to stimulate recombination in genomic regions that are normally refractory to genetic variation in key crop species. Developing ways to increase recombination rates in genome areas of low recombination will contribute to more efficient breeding. The outcome will allow reducing the time required to develop new elite germplasm and enable rapid introgression of desirable genes from diverse sources to facilitate developing more robust crops that will exhibit superior productivity in sustainable ways and be better suited to deal with the challenges arising from the climate change. The goal of this project is to gain an understanding of how DNA methylation influences the meiotic recombination landscape in plants. Meiotic recombination is initiated by the formation of numerous programmed double-strand breaks in chromosomal DNA, a small proportion of which are processed to form crossovers. Thus, genetic variation generated by a single round of meiotic recombination is limited. Furthermore, the distribution of crossovers in plants with large genomes, which include most crops, is highly biased towards the ends of chromosomes. As a result, extensive interstitial and centromere proximal chromosome regions rarely recombine. Yet, these large genome areas contain roughly one-fifth of the genes in maize and even larger gene fractions in some other crops, which presents a serious impediment to plant breeding. Recent studies indicate that DNA methylation is a critical factor shaping crossover landscapes. However, the exact relationship between recombination and DNA methylation is not understood. This project seeks to elucidate how DNA methylation affects recombination patterns at the mechanistic level and lay foundations for methods to control crossover landscapes in crops by altering DNA methylation. The study will be conducted in maize and Brassica rapa, to explore the behavior of both monocot and dicot genomes. To elucidate the effect of DNA methylation on meiotic recombination, a protocol to transiently alter DNA methylation patterns in meiosis will be developed. The chemically demethylated plants and selected mutants defective in DNA methylation will be used to determine which steps and processes of meiosis are altered by DNA methylation. It will be also established which specific aspect of DNA methylation affects crossover landscape.
该联盟计划开发高度创新的策略,以刺激关键作物物种中通常难以遗传变异的基因组区域的重组。开发提高低重组基因组区域重组率的方法将有助于提高育种效率。这一成果将减少开发新的优质种质所需的时间,并使来自不同来源的理想基因能够快速渗入,以促进开发更健壮的作物,这些作物将以可持续的方式表现出卓越的生产力,并更适合应对气候变化带来的挑战。该项目的目标是了解DNA甲基化如何影响植物减数分裂重组景观。减数分裂重组是由染色体DNA中大量程序性双链断裂的形成引发的,其中一小部分被加工成交叉。因此,由单轮减数分裂重组产生的遗传变异是有限的。此外,在具有大基因组的植物(包括大多数作物)中,杂交的分布高度偏向于染色体末端。因此,广泛的间质和着丝粒近端染色体区域很少重组。然而,这些大的基因组区域包含了大约五分之一的玉米基因,甚至在其他一些作物中包含了更大的基因部分,这对植物育种构成了严重的障碍。最近的研究表明,DNA甲基化是形成交叉景观的关键因素。然而,重组和DNA甲基化之间的确切关系尚不清楚。该项目旨在阐明DNA甲基化如何在机制水平上影响重组模式,并为通过改变DNA甲基化来控制作物交叉景观的方法奠定基础。该研究将在玉米和油菜中进行,以探索单子叶和双子叶基因组的行为。为了阐明DNA甲基化对减数分裂重组的影响,将开发一种瞬时改变减数分裂中DNA甲基化模式的方案。化学去甲基化植物和DNA甲基化缺陷的选择突变体将用于确定DNA甲基化改变减数分裂的哪些步骤和过程。还将确定DNA甲基化的哪个特定方面影响交叉景观。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Role of DNA Topoisomerase Binding Protein 1 (TopBP1) in Genome Stability in Arabidopsis.
DNA拓扑异构酶结合蛋白1(TOPBP1)在拟南芥中基因组稳定性中的作用。
  • DOI:
    10.3390/plants10122568
  • 发表时间:
    2021-11-24
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Parra-Nunez P;Cooper C;Sanchez-Moran E
  • 通讯作者:
    Sanchez-Moran E
Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.
  • DOI:
    10.3389/fpls.2021.631323
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    5.6
  • 作者:
    Osman K;Algopishi U;Higgins JD;Henderson IR;Edwards KJ;Franklin FCH;Sanchez-Moran E
  • 通讯作者:
    Sanchez-Moran E
Meiotic chromosome axis remodelling is critical for meiotic recombination in Brassica rapa.
  • DOI:
    10.1093/jxb/erab035
  • 发表时间:
    2021-04-02
  • 期刊:
  • 影响因子:
    6.9
  • 作者:
    Cuacos M;Lambing C;Pachon-Penalba M;Osman K;Armstrong SJ;Henderson IR;Sanchez-Moran E;Franklin FCH;Heckmann S
  • 通讯作者:
    Heckmann S
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Eugenio Sanchez-Moran其他文献

Eugenio Sanchez-Moran的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Eugenio Sanchez-Moran', 18)}}的其他基金

MEIAD: Investigating roles for Meiosis Associated Degradation during meiotic recombination in plants
MEIAD:研究植物减数分裂重组过程中减数分裂相关降解的作用
  • 批准号:
    BB/Y002512/1
  • 财政年份:
    2023
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Research Grant
16 ERA-CAPs: Meiotic recombination in plants: controlling the transition of DNA double-strand breaks to genetic crossovers (MEIOREC).
16 ERA-CAP:植物减数分裂重组:控制 DNA 双链断裂到遗传交叉的转变 (MEIOREC)。
  • 批准号:
    BB/S00467X/1
  • 财政年份:
    2018
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Research Grant
Integrating chromatin structure and global chromosome dynamics
整合染色质结构和整体染色体动力学
  • 批准号:
    BB/F02391X/1
  • 财政年份:
    2008
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Fellowship

相似海外基金

18-BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production
18-BTT EAGER:用于生产单倍体诱导系和细胞质雄性不育双单倍体的系统,用于高效杂交生产
  • 批准号:
    BB/S019995/1
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Research Grant
BTT EAGER: Investigating Genetic, Epigenetic and Environmental Control of Meiotic Recombination using Fluorescent Crossover Reporters in Tomato
BTT EAGER:利用番茄荧光交叉报告基因研究减数分裂重组的遗传、表观遗传和环境控制
  • 批准号:
    1844264
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Continuing Grant
BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production
BTT EAGER:用于生产单倍体诱导系和细胞质雄性不育双单倍体的系统,用于高效杂交生产
  • 批准号:
    1844304
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Standard Grant
BTT EAGER: Cell type-specific profiling 2.0: Capturing subpopulations of cells undergoing a response
BTT EAGER:细胞类型特异性分析 2.0:捕获正在发生反应的细胞亚群
  • 批准号:
    1844803
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Standard Grant
BTT EAGER: Controlling meiotic recombination in crops by manipulating DNA methylation
BTT EAGER:通过操纵 DNA 甲基化控制作物减数分裂重组
  • 批准号:
    1844588
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Standard Grant
Collaborative Research: BTT EAGER: A wearable plant sensor for real-time monitoring of sap flow and stem diameter to accelerate breeding for water use efficiency
合作研究:BTT EAGER:一种可穿戴植物传感器,用于实时监测树液流量和茎直径,以加速育种,提高水分利用效率
  • 批准号:
    1844707
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Continuing Grant
18 BTT EAGER - Engineering complex traits using targeted, multiplexed genetic and epigenetic mutagenesis
18 BTT EAGER - 使用定向、多重遗传和表观遗传诱变设计复杂性状
  • 批准号:
    BB/S020853/1
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Research Grant
BTT EAGER: Clean genome editing through the use of nonintegrating T-DNA technology
BTT EAGER:通过使用非整合 T-DNA 技术进行清洁基因组编辑
  • 批准号:
    1848434
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Continuing Grant
BTT EAGER: Plant genome editing and engineering via novel nanotechnology-based systems
BTT EAGER:通过基于纳米技术的新型系统进行植物基因组编辑和工程
  • 批准号:
    1844701
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Continuing Grant
BTT EAGER: Mitotic Recombination between Homologous Chromosomes
BTT EAGER:同源染色体之间的有丝分裂重组
  • 批准号:
    1844705
  • 财政年份:
    2019
  • 资助金额:
    $ 25.79万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了