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Fabrication of Biopolymer Microarrays for SPR Imaging Measurements

用于 SPR 成像测量的生物聚合物微阵列的制造

基本信息

批准号：
0133151
负责人：
Robert Corn
金额：
$ 66.1万
依托单位：
University of Wisconsin-Madison
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2002
资助国家：
美国
起止时间：
2002-03-15 至 2004-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0133151&HistoricalAwards=false
关键词：
Fabrication Biopolymer Microarrays SPR Imaging

项目摘要

In research supported by the Analytical and Surface Chemistry and the Instrument Development in Biological Research Programs, Professor Robert M. Corn and his coworkers at the University of Wisconsin are developing new fabrication methods and spectroscopic detection techniques for biopolymer arrays on gold thin films. A combination of novel attachment chemistries and microfluidic delivery strategies are being employed to create high fidelity biosensor arrays of DNA, polypeptide and proteins on gold surfaces. Surface plasmon resonance imaging is a surface-sensitive detection technique that is being developed for the label-free detection of bioaffinity interactions in an array format. The DNA and protein arrays fabricated in this research effort will be used in conjunction with the surface plasmon resonance imaging methods for the high throughput study of protein-DNA and protein-protein interactions.Professor Robert M. Corn and his coworkers at the University of Wisconsin are creating high quality biochips that consist of DNA, polypeptides, and proteins attached onto gold thin film surfaces in a multi-element array format. By detecting the binding of other biomolecules onto these biochips with the spectroscopic technique of surface plasmon resonance imaging, these biopolymer arrays can be used as multi-element biosensors in the areas of environmental sensing and medical diagnostics. In addition to the detection of biological compounds, the high throughput study of bioaffinity interactions with these arrays will have immediate applications in the areas of gene expression and proteomics

在由分析和表面化学和生物研究计划中的仪器开发支持的研究中，罗伯特M。玉米和他在威斯康星州大学的同事正在开发新的制造方法和光谱检测技术，用于在金薄膜上的生物聚合物阵列。新型附着化学和微流体递送策略的组合被用于在金表面上创建DNA、多肽和蛋白质的高保真度生物传感器阵列。表面等离子体共振成像是一种表面敏感的检测技术，正在开发用于阵列形式的生物亲和相互作用的无标记检测。在这项研究工作中制造的DNA和蛋白质阵列将与表面等离子体共振成像方法结合使用，用于蛋白质-DNA和蛋白质-蛋白质相互作用的高通量研究。玉米和他在威斯康星州大学的同事们正在创造高质量的生物芯片，这种芯片由DNA、多肽和蛋白质组成，以多元素阵列的形式附着在金薄膜表面。通过表面等离子体共振成像的光谱技术检测其他生物分子在这些生物芯片上的结合，这些生物聚合物阵列可以用作环境传感和医学诊断领域的多元素生物传感器。除了检测生物化合物外，利用这些阵列进行的生物亲和相互作用的高通量研究将在基因表达和蛋白质组学领域中具有直接的应用