Development of SPR Phase Imaging and SPR Diffraction Methods for Ultrasensitive Microarray Biosensing

用于超灵敏微阵列生物传感的 SPR 相位成像和 SPR 衍射方法的开发

• 批准号: 1057638
• 负责人: Robert Corn
• 金额: $ 46.83万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1057638&HistoricalAwards=false
• 关键词: Development; SPR; Phase; Imaging; Diffraction

The Chemical Measurement and Imaging program (CMI) of the Division of Chemistry supports Professor Robert M. Corn and his group at the University of California-Irvine to develop new instrumentation and methodologies for the label-free detection of biologically relevant molecules at very low concentrations. Specifically, two new optical biosensing techniques are being developed: surface plasmon resonance (SPR) phase imaging and optical diffraction methods from nanowire arrays, nanogaps, and nanostructured interfaces. These two new detection methods use the inherent refractive index of biomolecules as they bind to biosensor surfaces, and require both the implementation of high fidelity surface chemistries for the fabrication of well-characterized bioactive nanostructured surfaces, and the creation of novel optical detection techniques such and SPR-enhanced phase gratings and optical diffraction. These biosensor surfaces will be used in conjunction with microfluidic microarrays to create a multiplexed biosensor format to detect and identify minute quantities of multiple DNAs, RNAs, and proteins at concentrations relevant for many biomedical applications such as discovery of new cancer and cardiac biomarkers.The development of low cost, easy-to-use biosensors is a key to improving health. The refractive index-based methods being developed by Prof. Corn may offer fast, simple, and relatively inexpensive multiplexed bioassays. The research impacts the education of graduate students and postdoctoral scholars, including members of underrepresented groups, across a variety of related scientific disciplines including biomedical engineering, genomics, and materials science. Undergraduates and high school students who participate in this research are introduced to scientific research as a means to create a better world, and will become aware of how low-cost biosensing can impact community health both locally and globally.

化学测量和成像计划（CMI）的化学部支持教授罗伯特M。玉米和他的团队在加州大学欧文分校开发新的仪器和方法，用于在非常低的浓度下无标记检测生物相关分子。具体而言，两种新的光学生物传感技术正在开发中：表面等离子体共振（SPR）相位成像和光学衍射方法从纳米线阵列，纳米间隙，和纳米结构的接口。这两种新的检测方法使用生物分子的固有折射率，因为它们结合到生物传感器表面，并需要高保真表面化学的实施，用于制造良好表征的生物活性纳米结构表面，并创建新的光学检测技术，如SPR增强相位光栅和光学衍射。这些生物传感器表面将与微流控微阵列结合使用，以创建一个多路复用的生物传感器格式，以检测和识别微量的多种DNA，RNA和蛋白质，其浓度与许多生物医学应用相关，例如发现新的癌症和心脏生物标志物。开发低成本，易于使用的生物传感器是改善健康的关键。Corn教授正在开发的基于折射率的方法可以提供快速，简单和相对便宜的多重生物测定。这项研究影响了研究生和博士后学者的教育，包括代表性不足的群体的成员，跨越各种相关的科学学科，包括生物医学工程，基因组学和材料科学。参与这项研究的本科生和高中生将被引入科学研究，作为创造更美好世界的手段，并将意识到低成本的生物传感如何影响当地和全球的社区健康。