Enzymatically Amplified SPRI Biosensing of DNA, RNA and Protein Microarrays

DNA、RNA 和蛋白质微阵列的酶放大 SPRI 生物传感

• 批准号: 0551935
• 负责人: Robert Corn
• 金额: --
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0551935&HistoricalAwards=false
• 关键词: Enzymatically; Amplified; SPRI; Biosensing; DNA

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Robert Corn of the University of California at Irvine. Prof. Corn's research program focuses on the development of ultra sensitive biosensor micro arrays based on surface plasmon resonance imaging (SPRI) microscopy and spectroscopy. Surface biomolecular interactions and surface enzyme reactions will be utilized to either enhance the specificity or amplify the sensitivity of the biosensor micro arrays. The SPRI biosensor arrays will target DNA and RNA oligonucleotides and used to detect specific sequences in unlabelled DNA or messenger RNA samples and single base mismatches. The SPRI biosensor arrays will also be used for studying RNA-protein interactions. The research of Prof. Corn will integrate measurement science with problems that are currently at the frontier of biological research and will serve as an ideal platform for inter-disciplinary education. It will provide an excellent educational opportunity for trainees desiring work at the interface of chemistry and biology.The SPRI biosensor micro arrays developed in Prof. Corn's laboratory will result in significantly improved genomic analysis. The biosensor arrays could be used in various biomedical applications including studying gene expression patterns, diagnostic screening of genetic abnormalities and pathogen identification and detection.

化学部的分析和表面化学（ASC）计划将支持位于欧文的加州大学的Robert Corn教授的研究计划。Corn教授的研究项目侧重于基于表面等离子体共振成像（SPRI）显微镜和光谱学的超灵敏生物传感器微阵列的开发。 表面生物分子相互作用和表面酶反应将用于增强特异性或放大生物传感器微阵列的灵敏度。 SPRI生物传感器阵列将靶向DNA和RNA寡核苷酸，并用于检测未标记DNA或信使RNA样品中的特定序列和单碱基错配。 SPRI生物传感器阵列也将用于研究RNA-蛋白质相互作用。 玉米教授的研究将测量科学与目前生物研究前沿的问题相结合，并将成为跨学科教育的理想平台。 这将为希望从事化学和生物学接口工作的学员提供一个极好的教育机会。玉米教授实验室开发的SPRI生物传感器微阵列将显著改善基因组分析。 该生物传感器阵列可用于各种生物医学应用，包括研究基因表达模式、遗传异常的诊断筛选以及病原体的鉴定和检测。