CAREER: Effects of Interstitial Flow on Extracellular Matrix Architecture

职业：间质流对细胞外基质结构的影响

• 批准号: 0134551
• 负责人: Melody Swartz
• 金额: $ 37.5万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至 2007-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0134551&HistoricalAwards=false
• 关键词: CAREER; Effects; Interstitial; Flow; Extracellular

0134551SwartzUnder this CAREER Award, macroscale tissue mechanics will be bridged to cellular- and molecular-level control elements to examine how cells act in concert to manage mechanical stresses in their shared environment. The focus is on issues of tissue fluid balance, in particular on the effects of interstitial flow on matrix remodeling in a dynamic living environment. To characterize the system, an in vivo model of lymphangiogenesis into an implanted collagen gel (in situ model in the rat tail) will be observed over time. Based on the qualitative data obtained, a 3D in vitro model in which the physical environment can be tightly controlled and cell response readily ascertained will be developed. The two models will then be used symbiotically to examine the relationship between extracellular matrix architecture and interstitial flow, with particular focus on the organization and development of interstitial water channels. The notion of interstitial-flow induced matrix remodeling and vessel formation can be used to address a number of related research questions.The educational component focuses on integrating traditional biomedical engineering topics with contemporary problems in the biological sciences and biotechnology. Specific areas to be addressed are: reconstructing the biomechanics and biotransport curriculum within the department (new courses in Advanced Tissue Mechanics and Transport in Living Systems and increased involvement in the NSF Engineering Research Center on Engineering Education), enhancing the bioengineering community within the engineering school (establish a focus in biomechanics) and introducing non-traditional, interdisciplinary components to the engineering curriculum (new course in societal implications of technology).

0134551Swartz在这个CAREER奖下，宏观组织力学将被桥接到细胞和分子水平的控制元素，以研究细胞如何协同作用，以管理其共享环境中的机械应力。 重点是组织液平衡的问题，特别是在一个动态的生活环境中基质重塑间质流的影响。 为了表征该系统，将随时间观察淋巴管生成到植入的胶原凝胶中的体内模型（大鼠尾部中的原位模型）。 基于所获得的定性数据，将开发一种3D体外模型，在该模型中，可以严格控制物理环境，并且可以容易地确定细胞反应。 然后，这两个模型将被用于共生检查细胞外基质结构和间质流之间的关系，特别关注的组织和发展的间质水通道。 血流诱导基质重塑和血管形成的概念可用于解决一些相关的研究问题。教育部分侧重于将传统的生物医学工程主题与生物科学和生物技术中的当代问题相结合。 需要处理的具体领域是：在系内重建生物力学和生物运输课程（高级组织力学和生命系统运输的新课程，并增加了对NSF工程教育工程研究中心的参与），加强了工程学院内的生物工程社区（建立生物力学的重点），并引入非传统的，跨学科的组成部分，工程课程（新课程的社会影响的技术）。