CAREER: Advanced Single Molecule Techniques on DNA-Protein Interactions

职业：DNA-蛋白质相互作用的先进单分子技术

• 批准号: 0134916
• 负责人: Taekjip Ha
• 金额: $ 55万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0134916&HistoricalAwards=false
• 关键词: CAREER; Advanced; Single; Molecule; Techniques

The scientific component of this project focuses on the development and utilization of advanced single-molecule techniques to study the fundamentals of DNA-protein interactions. Methods such as x-ray crystallography and nuclear magnetic resonance have provided a vast array of structural detail for biological molecules, yet are largely limited to a static view that is based on ensemble measurements. In this work single molecule fluorescence detection and spectroscopy will be combined with nano-mechanical manipulations, bio-friendly surface engineering, and site-directed mutagenesis to construct suitable DNA structures and engineered proteins and to study their dynamic interactions at the single molecule level. The educational component of the project focuses on the expansion of the physics undergraduate program at the university through a course that will introduce students in physics, chemistry, bioengineering, and biology to both traditional and modern techniques in biophysics. The course will have a strong laboratory component that will be integrated with the university's REU program and a new departmental senior thesis program. Students will be introduced to some of the latest biophysical techniques, including patch clap, atomic force microscopy, optical tweezers, magnetic tweezers, and single molecule fluorescence detection and spectroscopy. This project is jointly funded by the Division of Physics and the Office of Multidisciplinary Activities in the Directorate for Mathematical and Physical Sciences and by the Division of Molecular and Cellular Biosciences in the Directorate for Biological Sciences.

该项目的科学部分侧重于开发和利用先进的单分子技术，以研究DNA-蛋白质相互作用的基本原理。 X射线晶体学和核磁共振等方法为生物分子提供了大量的结构细节，但在很大程度上限于基于系综测量的静态视图。 在这项工作中，单分子荧光检测和光谱将与纳米机械操作，生物友好的表面工程，和定点诱变相结合，以构建合适的DNA结构和工程蛋白质，并在单分子水平上研究它们的动态相互作用。 该项目的教育部分侧重于通过一门课程扩展大学的物理本科课程，该课程将向物理，化学，生物工程和生物学的学生介绍生物物理学中的传统和现代技术。 该课程将有一个强大的实验室组成部分，将与大学的REU计划和新的部门高级论文计划相结合。 学生将被介绍到一些最新的生物物理技术，包括补丁拍手，原子力显微镜，光镊，磁镊，单分子荧光检测和光谱。 该项目由数学和物理科学局物理司和多学科活动办公室以及生物科学局分子和细胞生物科学司共同资助。