Microbial Genome Seqencing: A Genomic Approach to Sulfur Biotransformations in the Ocean: The Genome Sequence of a Marine Roseobacter

微生物基因组测序：海洋中硫生物转化的基因组方法：海洋玫瑰杆菌的基因组序列

• 批准号: 0135210
• 负责人: Mary Ann Moran
• 金额: --
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0135210&HistoricalAwards=false
• 关键词: Microbial; Genome; Seqencing; Genomic; Approach

The emission of dimethylsulfide (DMS) from the ocean represents the major natural input of sulfur to the Earth's atmosphere. Once in the atmosphere, DMS is rapidly oxidized, forming sulfur aerosols that have an important impact on atmospheric chemistry and the global climate system. Recent evidence points to a key role for members of the marine roseobacter lineage in carrying out these globally-important functions. Representatives of the roseobacter group, including Silicibacter sp. DSS-3, are known to carry out many, if not most, of the major biogeochemical transformations of DMS and its precursor compound, dimethylsulfonioproionate (DMSP). Of particular importance is the fact that organisms like DSS-3 have the ability to produce DMS from DMSP but also the ability to metabolize DMSP by an alternative pathway that bypasses DMS altogether. Little is currently known about how these different pathways are regulated in marine bacteria, or how pathway regulation affects the complex DMS biogeochemistry in the sea. Thus the genome sequence of Silicibacter sp. DSS-3 will provide key information for deciphering how marine bacterioplankton control sulfur emission from the ocean. In this project, a fully closed and annotated sequence of Silicibacter sp. DSS-3 will be obtained, with the goal of developing molecular tools that can be used to investigate the microbiology and ecology of bacterially mediated organic sulfur transformations in the ocean. The roseobacter group is currently the only major lineage of marine heterotrophs whose members can be readily cultured and manipulated in the laboratory. As such, a genome sequence of Silicibacter sp. DSS-3 will also be of broad interest to marine biogeochemists and microbial ecologists. Sequence data will be provided to the scientific community through the TIGR Microbial Database site (www.tigr.org/tdb/tdb.html). Initial release of the sequence data will be in the form of contigs assembled at 3-fold sequence coverage, with monthly data releases thereafter. This is a Microbial Genome Sequencing Award funded through a collaborative activity between the National Science Foundation and the Department of Agriculture Initiative for Future Agriculture and Food Systems.

海洋排放的二甲基硫（DMS）是硫进入地球大气的主要自然输入。二甲硫醚一旦进入大气，就会被迅速氧化，形成硫气溶胶，对大气化学和全球气候系统产生重要影响。最近的证据表明，海洋蔷薇科的成员在执行这些全球重要功能方面发挥着关键作用。蔷薇科细菌的代表，包括Silicibacter sp. DSS-3，已知进行DMS及其前体化合物二甲基磺基丙酸盐（DMSP）的许多（如果不是大多数的话）主要生物地球化学转化。特别重要的是，像DSS-3这样的生物体具有从DMSP产生DMS的能力，而且还具有通过完全绕过DMS的替代途径代谢DMSP的能力。 目前对海洋细菌中这些不同的途径是如何调节的，或者途径调节如何影响海洋中复杂的DMS地球化学知之甚少。因此，Silicibacter sp. DSS-3的基因组序列将为破译海洋浮游细菌如何控制海洋硫排放提供关键信息。在该项目中，将获得Silicibacter sp. DSS-3的完全封闭和注释序列，目标是开发可用于研究海洋中细菌介导的有机硫转化的微生物学和生态学的分子工具。蔷薇类群是目前海洋异养生物中唯一的主要类群，其成员可以在实验室中容易地培养和操纵。因此，Silicibacter sp. DSS-3的基因组序列也将引起海洋生物化学家和微生物生态学家的广泛兴趣。序列数据将通过TIGR微生物数据库网站（www.tigr.org/tdb/tdb.html）提供给科学界。序列数据的初始发布将以3倍序列覆盖率组装的重叠群的形式进行，此后每月发布数据。 这是一个微生物基因组测序奖，由美国国家科学基金会和农业部未来农业和食品系统倡议之间的合作活动资助。