Metabolic Currencies of the Ocean Carbon Cycle

海洋碳循环的代谢货币

• 批准号: 1656311
• 负责人: Mary Ann Moran
• 金额: $ 74.92万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656311&HistoricalAwards=false
• 关键词: Metabolic; Currencies; Ocean; Carbon; Cycle

The roles of microbes in cycling carbon and nutrients in the ocean ? the largest biological system on Earth ? were initially described about 40 years ago. Now, it is known that half of Earth's primary production is carried out by marine phytoplankton, and half of that is recycled within weeks by marine bacteria. This proposal is a collaboration between microbiologists and chemists to identify the specific compounds that pass between phytoplankton and bacteria in surface ocean waters. Identifying the key chemicals of the ocean's microbial food web will provide insights into how the marine carbon cycle is regulated, generate data to improve ocean carbon models, and train new scientists at the interface of microbiology and chemistry. Hands-on learning opportunities in microbial ecology will be provided for high school students, both in the classroom and in marine ecosystems of the Georgia coast. Phytoplankton metabolites that sustain the flow of carbon between microbial autotrophs and heterotrophs in the surface ocean carbon cycle will be identified in this project. A matrix of model systems consisting of bacteria-phytoplankton co-cultures will be used as biological assays for key metabolites based on expression patterns of bacterial transporter genes. The chemical identity of candidate metabolites and evaluation of their potential ecological role will be carried out by exometabolomic analysis of co-cultures with bacterial transporter mutants. Both advanced mass spectrometry and NMR will be used for metabolomics analysis, taking advantage of the sensitivity and compound identification strengths of each. The distribution of candidate metabolites in the ocean microbiome and other microbial systems will be characterized by mining environmental sequence datasets for orthologous transporter genes. This project represents a novel approach to identifying metabolites important in microbiome function, compounds often difficult to address with standard chemical approaches because of their low concentrations and high biological demand.

微生物在海洋碳和营养物质循环中的作用？地球上最大的生物系统最初是在40年前被描述的现在，人们知道地球上一半的初级生产是由海洋浮游植物进行的，其中一半在几周内被海洋细菌回收。这项提议是微生物学家和化学家之间的合作，以确定在海洋表面沃茨中浮游植物和细菌之间传递的特定化合物。确定海洋微生物食物网的关键化学物质将提供有关海洋碳循环如何调节的见解，生成数据以改进海洋碳模型，并培养微生物学和化学界面的新科学家。将为高中学生提供微生物生态学的实践学习机会，无论是在课堂上还是在格鲁吉亚海岸的海洋生态系统中。本项目将确定浮游植物的代谢物，这些代谢物在海洋表层碳循环中维持微生物自养生物和异养生物之间的碳流动。由细菌-浮游植物共培养物组成的模型系统矩阵将被用作基于细菌转运蛋白基因表达模式的关键代谢物的生物测定。候选代谢物的化学鉴别及其潜在生态作用的评价将通过与细菌转运蛋白突变体共培养物的外代谢组学分析进行。先进的质谱法和NMR都将用于代谢组学分析，利用各自的灵敏度和化合物鉴定优势。候选代谢物在海洋微生物组和其他微生物系统中的分布将通过挖掘环境序列数据集来确定直链转运蛋白基因的特征。该项目代表了一种识别微生物组功能中重要代谢物的新方法，这些化合物通常难以用标准化学方法处理，因为它们的浓度低，生物需求高。

项目成果

Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes

• DOI: 10.1111/1462-2920.14560
• 发表时间: 2019-05-01
• 期刊: ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 5.1
• 作者: Nowinski, Brent;Motard-Cote, Jessie;Moran, Mary Ann
• 通讯作者: Moran, Mary Ann

Niche dimensions of a marine bacterium are identified using invasion studies in coastal seawater

• DOI: 10.1038/s41564-020-00851-2
• 发表时间: 2021-01
• 期刊: Nature Microbiology
• 影响因子: 28.3
• 作者: Brent Nowinski;M. Moran

Quantification of Amine- and Alcohol-Containing Metabolites in Saline Samples Using Pre-extraction Benzoyl Chloride Derivatization and Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry (UHPLC MS/MS)

使用预提取苯甲酰氯衍生化和超高效液相色谱串联质谱 (UHPLC MS/MS) 定量盐水样品中的胺和醇代谢物

• DOI: 10.1021/acs.analchem.0c03769
• 发表时间: 2021
• 期刊: Analytical Chemistry
• 影响因子: 7.4
• 作者: Widner, Brittany;Kido Soule, Melissa C.;Ferrer-González, Frank Xavier;Moran, Mary Ann;Kujawinski, Elizabeth B.
• 通讯作者: Kujawinski, Elizabeth B.