Direct Probing and Modeling of Adsorbed Layers of Surfactant/Lipid/Protein Mixtures atT Air/Water Interfaces

空气/水界面表面活性剂/脂质/蛋白质混合物吸附层的直接探测和建模

• 批准号: 0135317
• 负责人: Elias Franses
• 金额: $ 30万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0135317&HistoricalAwards=false
• 关键词: Direct; Probing; Modeling; Adsorbed; Layers

AbstractCTS-0135317Franses, Elias IPurdue University"Direct Probing and Modeling of Adsorbed Layers of Surfactant/Lipid/Protein Mixtures at Air/Water Interfaces" The main objectives are to understand quantitatively and qualitatively how surfactants, lipids, and proteins adsorb at air/water interfaces. By adsorbing, alone or in competition, surface active molecules modify the equilibrium and dynamic surface tension, the surface charges, the foaming behavior, and the protein denaturation behavior. These phenomena have important applications in bioprocessing of biological andbiochemical dispersions, spray drying of pharmaceutical solutions, spreading of agricultural chemical in leaves; also in foaming stability, and foam-based separations of dilute solutions of surfactants and proteins, mineral ore flotation, and detergency. A major focus is the area of replacement therapy of lung surfactant, which is a mixture of lipids and proteins responsible for stabilizing the lung alveoli and controlling breathing.The equilibrium and dynamic adsorption is probed not only with indirect surface tension methods but with direct optical (ellipsometry, EL) and infrared reflection absorption spectroscopy (IRRAS). IRRAS is used primarily to determine the extent, rates, and mechanisms of individual components. IRRAS, in combination with EL and surface tensiometry, has proven to be a valuable tool for quantitative probing of complexindustrial or biological mixtures. The results are used to develop formulations which are technically effective, cost-effective, and biologically or environmentally friendly; and to develop new, potentially effective lung surfactant formulations. The systems examined are bovine serum albumin, fibrinogen, various lipids and soaps, and model ionic surfactants. The data are used to guide quantitative modeling of diffusion/adsorption processes of surfactants, lipids, and proteins from the bulk solution or dispersion to the interface. The models are used to help design new improved formulations, and to predict how formulations may function in different solutions and different geometries.

摘要CTS-0135317 Franses，Elias IPurdue大学“空气/水界面处表面活性剂/脂质/蛋白质混合物的吸附层的直接探测和建模”主要目标是定量和定性地了解表面活性剂、脂质和蛋白质如何在空气/水界面处吸附。 通过单独或竞争吸附，表面活性分子改变平衡和动态表面张力、表面电荷、发泡行为和蛋白质变性行为。这些现象在生物和生物化学分散体的生物处理、药物溶液的喷雾干燥、农药在叶子中的扩散、泡沫稳定性以及表面活性剂和蛋白质的稀溶液的基于泡沫的分离、矿物浮选和去污中具有重要的应用。 一个主要的焦点是肺表面活性物质的替代治疗领域，肺表面活性物质是负责稳定肺泡和控制呼吸的脂质和蛋白质的混合物。平衡和动态吸附不仅用间接表面张力法，而且用直接光学（椭圆偏振法，EL）和红外反射吸收光谱法（IRRAS）进行探测。IRRAS主要用于确定单个组分的程度、速率和机制。 IRRAS结合EL和表面张力测量，已被证明是一个有价值的工具，用于定量探测复杂的工业或生物混合物。研究结果用于开发技术上有效、成本效益高、生物或环境友好的制剂;并开发新的、潜在有效的肺表面活性剂制剂。检查的系统是牛血清白蛋白，纤维蛋白原，各种脂质和肥皂，和模型离子表面活性剂。这些数据用于指导定量建模的扩散/吸附过程的表面活性剂，脂质和蛋白质从本体溶液或分散体的界面。这些模型用于帮助设计新的改进配方，并预测配方如何在不同的溶液和不同的几何形状中发挥作用。