Direct Probing and Modeling of Adsorbed Layers of Surfactant/Lipid/Protein Mixtures atT Air/Water Interfaces

普渡大学的franes， Elias“空气/水界面表面活性剂/脂质/蛋白质混合物吸附层的直接探测和建模”的主要目标是定量和定性地了解表面活性剂，脂质和蛋白质如何在空气/水界面吸附。通过吸附，单独或竞争，表面活性分子改变平衡和动态表面张力，表面电荷，发泡行为和蛋白质变性行为。这些现象在生物和生化分散体的生物处理、药剂溶液的喷雾干燥、农药在叶片中的喷洒等方面都有重要的应用。也用于泡沫稳定性，以及泡沫基分离的表面活性剂和蛋白质的稀溶液，矿物矿石的浮选和去除率。一个主要的焦点是肺表面活性剂的替代治疗领域，它是一种脂质和蛋白质的混合物，负责稳定肺泡和控制呼吸。利用间接表面张力法、直接光学（椭偏仪）和红外反射吸收光谱（IRRAS）等方法研究了平衡态和动态吸附。iras主要用于确定单个组分的范围、速率和机制。iras与EL和表面张力测定相结合，已被证明是复杂工业或生物混合物定量探测的有价值的工具。研究结果用于开发技术上有效、成本效益高、生物或环境友好的配方；并开发新的，潜在有效的肺表面活性剂配方。系统检查牛血清白蛋白，纤维蛋白原，各种脂质和肥皂，和模型离子表面活性剂。这些数据用于指导表面活性剂、脂质和蛋白质从散装溶液或分散液到界面的扩散/吸附过程的定量建模。这些模型用于帮助设计新的改进配方，并预测配方在不同溶液和不同几何形状下的作用。

AbstractCTS-0135317Franses, Elias IPurdue University"Direct Probing and Modeling of Adsorbed Layers of Surfactant/Lipid/Protein Mixtures at Air/Water Interfaces" The main objectives are to understand quantitatively and qualitatively how surfactants, lipids, and proteins adsorb at air/water interfaces. By adsorbing, alone or in competition, surface active molecules modify the equilibrium and dynamic surface tension, the surface charges, the foaming behavior, and the protein denaturation behavior. These phenomena have important applications in bioprocessing of biological andbiochemical dispersions, spray drying of pharmaceutical solutions, spreading of agricultural chemical in leaves; also in foaming stability, and foam-based separations of dilute solutions of surfactants and proteins, mineral ore flotation, and detergency. A major focus is the area of replacement therapy of lung surfactant, which is a mixture of lipids and proteins responsible for stabilizing the lung alveoli and controlling breathing.The equilibrium and dynamic adsorption is probed not only with indirect surface tension methods but with direct optical (ellipsometry, EL) and infrared reflection absorption spectroscopy (IRRAS). IRRAS is used primarily to determine the extent, rates, and mechanisms of individual components. IRRAS, in combination with EL and surface tensiometry, has proven to be a valuable tool for quantitative probing of complexindustrial or biological mixtures. The results are used to develop formulations which are technically effective, cost-effective, and biologically or environmentally friendly; and to develop new, potentially effective lung surfactant formulations. The systems examined are bovine serum albumin, fibrinogen, various lipids and soaps, and model ionic surfactants. The data are used to guide quantitative modeling of diffusion/adsorption processes of surfactants, lipids, and proteins from the bulk solution or dispersion to the interface. The models are used to help design new improved formulations, and to predict how formulations may function in different solutions and different geometries.