Direct Probing and Modeling of Adsorbed Layers of Surfactant/Lipid/Protein Mixtures at Air/Water Interfaces and in Aqueous Solution

空气/水界面和水溶液中表面活性剂/脂质/蛋白质混合物吸附层的直接探测和建模

• 批准号: 0651942
• 负责人: Elias Franses
• 金额: --
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0651942&HistoricalAwards=false
• 关键词: Direct; Probing; Modeling; Adsorbed; Layers

Franses / Purdue / 0651942Intellectual Merit. The project examines how to understand and control the extent and rate of adsorption of surfactants, lipids, and proteins at air/water interfaces by focusing on the prevailing fundamental physicochemical, biophysical, and engineering phenomena and mechanisms. Such adsorption determines the equilibrium and dynamic surface tension, which affects free surface flows involving bubbles, drops, and liquid jets, and controls pressure and mass transfer in lung alveoli. Unique features include: (i) direct probing of the interfacial layers of aqueous solutions or dispersions of surfactants, lipids, proteins, and their mixtures, with quantitative infrared reflection absorption spectroscopy and ellipsometry; (ii) control of particulate or molecular adsorption of insoluble lipids by developing improved methods of lipid dispersion preparation; (iii) understanding of competitive adsorption of lipids and serum proteins (albumin or fibrinogen) which inhibit lung surfactant; (iii) studies of rates and mechanisms by which lipids produce very low dynamic surface tensions, less then 10 mN/m during surface compression; (iv) studies of mechanisms by which lipids can exclude or expel soluble proteins from the interfacial region; and (v) interactions of proteins with lipids in bulk solution and on surfaces, for developing biocompatible liposome formulations . The results are relevant to inkjet printing processes, agricultural sprays, DNA and protein microarrays, and processes affected by foam formation and stability. An important biomedical application, developing lipid formulations for long-term potential uses as lung surfactant replacement formulations, is an area of focus. The dynamic surface properties of lipid dispersions are related to the size and morphology of the dispersed particles, liposomes or vesicles. The scientific uniqueness of this project is the understanding of how to control lipid and protein adsorption at the air-water interface by controlling both their bulk and surface interactions. The unique aspect of the approach is the use of rigorous direct probes and quantitative engineering modeling principles in developing a rational understanding of lipid/protein interactions at fluid interfaces and in aqueous solution.Broader Impacts. Major societal benefits include: (i) understanding how to treat alveolar respiratory diseases of premature infants and adults with new formulations; and (ii) understanding how aggregation of liposomes or vesicles with serum proteins can be controlled by using a second lipid mixed with first so that aggregation with proteins is inhibited. The knowledge base for many of the above applications will be substantially expanded. The results may lead to partnerships with medical researchers or professionals, and with industrial and academic researchers dealing with such applications. This research aims at effective training of graduate and undergraduate students, who will be exposed to state-of-the-art experimentation and modeling. Certain results will be used in teaching of graduate and undergraduate courses. Developing and refining surface layer characterization will improve the infrastructure of research and education in interfacial, biomedical, biochemical, and mineral engineering. The P.I. is committed to diversity in recruiting graduate and undergraduate students for this project, and has worked with several members of under-represented groups over the past five years. He will recruit for minority students from the Purdue "Surf" program, in which minority seniors, from Purdue or other schools, are actively recruited, hired, mentored, and advised to pursue graduate or professional studies.

弗朗西斯/普渡大学/0651942智力优点。 该项目通过关注普遍的基本物理化学、生物物理和工程现象和机制，研究如何理解和控制表面活性剂、脂质和蛋白质在空气/水界面的吸附程度和速率。 这种吸附决定了平衡和动态表面张力，这会影响涉及气泡、液滴和液体射流的自由表面流动，并控制肺泡中的压力和质量传递。 独特的功能包括：（i）利用定量红外反射吸收光谱和椭圆光度法直接探测表面活性剂、脂质、蛋白质及其混合物的水溶液或分散体的界面层； (ii) 通过开发改进的脂质分散体制备方法来控制不溶性脂质的颗粒或分子吸附； (iii) 了解抑制肺表面活性物质的脂质和血清蛋白（白蛋白或纤维蛋白原）的竞争吸附； (iii) 研究脂质在表面压缩过程中产生极低动态表面张力（低于 10 mN/m）的速率和机制； (iv) 研究脂质可从界面区域排除或驱逐可溶性蛋白质的机制； (v)蛋白质与本体溶液中和表面上的脂质的相互作用，用于开发生物相容性脂质体制剂。 结果与喷墨印刷工艺、农用喷雾剂、DNA 和蛋白质微阵列以及受泡沫形成和稳定性影响的工艺相关。 一个重要的生物医学应用，即开发脂质制剂作为肺表面活性剂替代制剂的长期潜在用途，是一个重点领域。 脂质分散体的动态表面特性与分散颗粒、脂质体或囊泡的尺寸和形态有关。该项目的科学独特性在于了解如何通过控制脂质和蛋白质的体积和表面相互作用来控制空气-水界面上的脂质和蛋白质吸附。该方法的独特之处在于使用严格的直接探针和定量工程建模原理来合理理解流体界面和水溶液中的脂质/蛋白质相互作用。更广泛的影响。 主要社会效益包括：(i) 了解如何用新配方治疗早产儿和成人的肺泡呼吸道疾病； (ii)了解如何通过使用与第一种脂质混合的第二种脂质来控制脂质体或囊泡与血清蛋白的聚集，从而抑制与蛋白质的聚集。 上述许多应用的知识库将得到大幅扩展。研究结果可能会导致与医学研究人员或专业人士以及处理此类应用的工业和学术研究人员建立合作伙伴关系。这项研究旨在有效培训研究生和本科生，让他们接触最先进的实验和建模。某些成果将用于研究生和本科生课程的教学。 开发和完善表面层表征将改善界面、生物医学、生物化学和矿物工程方面的研究和教育基础设施。 P.I.致力于为该项目招收研究生和本科生的多元化，并在过去五年中与代表性不足群体的多名成员合作。他将从普渡大学的“冲浪”计划中招募少数族裔学生，在该计划中，来自普渡大学或其他学校的少数族裔高年级学生将被积极招募、雇用、指导，并建议他们攻读研究生或专业学习。