Characterization of Marine Carbon Monoxide Utilizing Bacteria

该项目的目标是通过最新的微生物学和分子方法相结合的方法，确定对沿海海洋环境中的CO氧化负有责任的微生物群落的组成成员，并估计它们对总的原位CO生物氧化的贡献。研究人员将利用一种不依赖液体分批培养的简单方法来筛选和分离具有特定表型的微生物。复制过滤器将允许分离和提纯活性共代谢菌落。由于这种方法在空间上分离了其他相互竞争的种群，因此可以同时恢复具有不同生长速度和营养需求的生物体，并且可以同时快速筛选大量细菌。将用14种时间序列的CO氧化方法测定选定菌株的细胞特异性CO氧化活性。在庞大且不断增长的16S数据库的背景下，基于16S-rRNA序列信息的分子系统发育将决定我们的培养计划导致的海洋一氧化碳氧化细菌的系统发育关系和特性。根据CO氧化生物的生理测量和丰度估计计算的集体CO氧化速率将与生物地球化学CO氧化测量相平衡，以验证CO氧化细菌的活动和丰度可以解释在自然水域中观察到的总CO氧化。

The goal of this project is to identify component members of the microbial community responsible for the oxidation of CO in coastal marine environments through a combination of recent microbiological and molecular approaches, and to estimate their contributions to total in situ CO bio-oxidation. The researchers will utilize a simple method that does not rely on liquid batch?cultures, for screening and isolating microorganisms with a particular phenotype. Replicate filters will allow for the isolation and purification of active CO-metabolizing colonies. Since this method spatially separates otherwise competing populations, simultaneous recovery of organisms with different growth rates and nutritional requirements is possible, and large numbers of bacteria can be screened rapidly at one time. Cell-specific CO-oxidation activity will be determined for selected strains with time-series 14 CO-oxidation methods. Molecular phylogeny based on 16S-rRNA sequence information within the context of the large and growing 16S database will determine the phylogenetic relatedness and identity of marine CO?oxidizing bacteria that result from our cultivation program. A collective CO-oxidation rate calculated from physiological measurements and abundance estimates of CO-oxidizing organisms will be balanced with biogeochemical CO-oxidation measurements to verify that activity and abundance of CO-oxidizing bacteria can account for the total CO-oxidation that is observed in natural waters.