Stress Induced Coral Mortality: The Role of Opportunistic Bacterial Infections

压力引起的珊瑚死亡：机会性细菌感染的作用

• 批准号: 0137748
• 负责人: Forest Rohwer
• 金额: $ 39.17万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2005-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0137748&HistoricalAwards=false
• 关键词: Stress; Induced; Coral; Mortality; Role

Coral reefs have declined dramatically over the last three decades. Several factors may have contributed to this process, including over-fishing, increasing CO2 levels, changes in sea surface temperatures, eutrophication, sedimentation, and pollutants. However the causal relationship between most of these stressors and reef decline has yet to be established. There has also been an alarming increase in coral disease incidence during the last two decades. The causes of the majority of these diseases remain unknown yet the diseases continue to cause massive die offs of corals. Of the 29 coral diseases that have been reported, only two pathogens have been isolated which fulfill Koch's postulates. Are some of the undiagnosed diseases caused by opportunistic, rather than specific, pathogens? Coral disease incidences and human activity are correlated. Again, the causal connection, if any, between these two observations is not known. Dr. Rohwer and associates suggest that corals exposed to anthropogenic and environmental stressors become susceptible to opportunistic infection resulting in coral mortality and disease. In preliminary studies, they have found that the microbial community normally living on corals consists of specific bacteria growing at a controlled rate. For example, corals living on reefs as far apart as Bermuda and Panama have characteristic microbiotas, that are specific to individual coral species. They have also observed that bacterial numbers and growth rates are very similar between healthy corals. The relationship between the coral and its associated microbial community rapidly changes when the coral is exposed to stressful environmental or anthropogenic conditions. In experiments, additions of organic nutrients rapidly lead to coral death, which was prevented by adding antibiotics. This strongly suggests that bacterial overgrowth is responsible for the stressor-induced mortality. Other investigators have shown that coral mortality caused by stressors, including sedimentation, crude oil, and copper sulfate, could be prevented using antibiotics. The investigators have also documented that both bacterial numbers and production rates increase by over an order of magnitude on diseased, as compared to healthy, corals. In this research, they will characterize the normal range of microbial growth parameters and prokaryotic diversity on healthy, unstressed M. franksi. They will then treat corals with a number of potential stressors to determine which kill corals by causing microbial overgrowth. Finally, they will monitor changes in bacterial growth and community composition that occurs with opportunistic bacterial infection. This research will improve our understanding of the relationship between a coral and its associated bacterial community, and how this relationship changes with stress and disease. This research will also provide a better understanding of the connection between human activities and the incidence of coral disease, providing important data for the conservation and management of coral reefs.

过去三十年来，珊瑚礁急剧减少。有几个因素可能促成了这一过程，包括过度捕捞、二氧化碳含量增加、海面温度变化、富营养化、沉积和污染物。然而，大多数这些压力源与珊瑚礁衰退之间的因果关系尚未确定。 在过去的二十年中，珊瑚疾病的发病率也出现了惊人的增长。大多数这些疾病的原因仍然未知，但这些疾病继续导致珊瑚大规模死亡。在已报道的 29 种珊瑚疾病中，仅分离出两种满足科赫假设的病原体。一些未确诊的疾病是由机会性病原体而不是特定病原体引起的吗？珊瑚疾病的发生率与人类活动相关。同样，这两个观察结果之间的因果关系（如果有的话）尚不清楚。 Rohwer 博士及其同事认为，暴露于人为和环境压力源的珊瑚容易受到机会性感染，导致珊瑚死亡和疾病。 在初步研究中，他们发现通常生活在珊瑚上的微生物群落由以受控速度生长的特定细菌组成。例如，生活在相距百慕大和巴拿马的珊瑚礁上的珊瑚具有特定于单个珊瑚物种的特征微生物群。他们还观察到健康珊瑚之间的细菌数量和生长速度非常相似。当珊瑚暴露在有压力的环境或人为条件下时，珊瑚与其相关微生物群落之间的关系会迅速发生变化。在实验中，添加有机营养物质会迅速导致珊瑚死亡，而添加抗生素可以防止这种情况发生。这强烈表明细菌过度生长是压力源引起的死亡的原因。其他研究人员表明，使用抗生素可以预防由沉积、原油和硫酸铜等压力源引起的珊瑚死亡。 研究人员还记录了与健康珊瑚相比，患病珊瑚的细菌数量和生产率增加了一个数量级以上。在这项研究中，他们将描述健康、无应激的弗兰克斯微生物的正常生长参数范围和原核生物多样性。然后，他们将用许多潜在的压力源来处理珊瑚，以确定哪些压力源会导致微生物过度生长而杀死珊瑚。最后，他们将监测机会性细菌感染引起的细菌生长和群落组成的变化。 这项研究将提高我们对珊瑚与其相关细菌群落之间关系的理解，以及这种关系如何随压力和疾病而变化。这项研究还将更好地了解人类活动与珊瑚疾病发生之间的联系，为珊瑚礁的保护和管理提供重要数据。