SGER: Custom Reagents for Flavonoid Analysis

SGER：用于类黄酮分析的定制试剂

• 批准号: 0140114
• 负责人: Virginia Walbot
• 金额: $ 4.98万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0140114&HistoricalAwards=false
• 关键词: SGER; Custom; Reagents; Flavonoid; Analysis

Flavonoids are metabolites produced exclusively by plants that are well known as the blue, red, and purple pigments in flowers, fruits, and leaves. As such, these compounds are important for attracting pollinators and seed dispersers. However, flavonoids are also used by plants for a large number of other purposes, such as protecting exposed tissues from UV radiation, as signaling molecules, and as part of the chemical arsenal against pathogens, predators, and other challenges. Although all higher plants synthesize flavonoids, these compounds clearly participate in distinct processes in different plants. Therefore, it is important to understand how flavonoids are synthesized in different plant species. Our laboratories study the organization and regulation of the flavonoid biosynthetic pathway in plant cells, how the products are transported to their final destination inside the cell, and how this system has developed over the course of plant evolution. Despite the success of genetic and biochemical analysis of flavonoid synthesis, current studies into detailed mechanisms are hampered by the extraordinarily expensive reagent costs and the lack of radiolabeled compounds, while analysis of enzyme levels and localization are confounded by the lack of adequate antibody reagents. This project is aimed at assembling a standardized collection of flavonoid compounds and antibodies. These reagents will be stored at specific locations for distribution among the group and, if feasible, to other publicly-funded groups working on flavonoid synthesis. The project will immediately impact the quality of the work that is done in our laboratories and at the same time open up entirely new avenues of investigation.

黄酮类化合物是由植物专门产生的代谢产物，众所周知是花、果实和叶中的蓝色、红色和紫色色素。 因此，这些化合物对于吸引传粉者和种子传播者是重要的。 然而，类黄酮也被植物用于大量其他目的，例如保护暴露的组织免受紫外线辐射，作为信号分子，以及作为对抗病原体，捕食者和其他挑战的化学武器库的一部分。 虽然所有高等植物都合成类黄酮，但这些化合物在不同植物中参与不同的过程。 因此，重要的是要了解黄酮类化合物是如何在不同的植物物种合成。 我们的实验室研究植物细胞中类黄酮生物合成途径的组织和调节，产品如何运输到细胞内的最终目的地，以及该系统如何在植物进化过程中发展。 尽管类黄酮合成的遗传和生物化学分析取得了成功，但目前对详细机制的研究受到非常昂贵的试剂成本和缺乏放射性标记化合物的阻碍，而酶水平和定位的分析由于缺乏足够的抗体试剂而受到混淆。 该项目旨在收集标准化的类黄酮化合物和抗体。 这些试剂将储存在特定的地点，以便在小组中分发，如果可行的话，分发给其他从事类黄酮合成的公共资助小组。 该项目将立即影响我们实验室所做工作的质量，同时开辟全新的研究途径。