X-ray Diffraction Laboratory Upgrade

X射线衍射实验室升级

• 批准号: 0200293
• 负责人: Janet Smith
• 金额: $ 29.76万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0200293&HistoricalAwards=false
• 关键词: ray; Diffraction; Laboratory; Upgrade

This award supports improvements in the capability and reliability of the X-ray diffraction laboratory used for study of single crystals of biological macromolecules at Purdue University. The equipment to be obtained with this support will be used for in research aimed at determination of the three-dimensional crystal structures of proteins, RNA molecules, and of protein-nucleic acid complexes. Knowledge of such structures are essential to molecular level understanding of how biological macromolecules function in a variety of processes. Three types of equipment will be obtained: a new diffraction instrument that will improve throughput and, by providing a more monochromatic and intense X-ray beam, that will facilitate the study of very large macromolecules. The new instrument will allow flash-frozen crystals to be saved in their frozen state for later diffraction experiments. A new optical system for an existing diffraction instrument will improve the intensity and quality of the X-ray beam, permitting more pricise measurements and use of smaller crystals than now possible. Finally a new microspectrophotometer will be obtained to monitor enzymatic reactions and measure ligand binding of crystalline macromolecules. The award provides improved infrastructure for one of the nation's leading academic laboratories in one of the most important areas of contemporary biology.

该奖项支持普渡大学用于研究生物大分子单晶的X射线衍射实验室的能力和可靠性的改进。通过这种支持获得的设备将用于旨在确定蛋白质、RNA分子和蛋白质-核酸复合物的三维晶体结构的研究。这些结构的知识是必不可少的分子水平的理解如何生物大分子在各种过程中的功能。 将获得三种类型的设备：一种新的衍射仪器，它将提高产量，并通过提供更单色和更强的X射线束，促进对非常大的高分子的研究。新仪器将允许快速冷冻晶体保存在其冻结状态，用于以后的衍射实验。现有衍射仪器的新光学系统将提高X射线束的强度和质量，允许更精确的测量和使用比现在更小的晶体。最后，一个新的显微分光光度计将获得监测酶反应和测量晶体大分子的配体结合。该奖项为当代生物学最重要领域之一的全国领先的学术实验室之一提供了更好的基础设施。