Molecular genetic basis of plant sensations to the diurnal environment

植物对昼夜环境感觉的分子遗传学基础

• 批准号: 157291638
• 负责人: Professor Dr. Seth J. Davis
• 金额: --
• 依托单位: Department of Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/157291638?language=en
• 关键词: Molecular; genetic; basis; plant; sensations

Plant growth and development requires robust detection of the diurnal environment. This occurs through a coupling mechanism of light detection to the circadian clock. We recently described the genetic loci that make the core of the oscillator. From this, we propose to further define the placement of additional circadian loci within the oscillator network, and to start to define the biochemical modes of action of these gene products. For this, we will test the hypothesis that a complex of ELF3 / ELF4 interacts with a photoreceptor and a transcription factor to mediate activation of clock genes; this is the dusk arm of the clock. In parallel, we will test if phosphorylation of TIC by AKIN10 directs protein stability of a coreclock protein; this is the dawn arm of the clock. These efforts integrate at a systems level through analyses of recently performed microarrays at the morning and dawn phases, and through the development of a revolutionary imaging technique that will describe when proteins interact within a living cell. The combined efforts of this proposal should radically elevate our mechanistic understanding of the plant circadian clock as it operates under natural diurnal conditions.

植物的生长和发育需要对昼夜环境进行强有力的检测。这是通过光检测与生物钟的耦合机制发生的。我们最近描述了构成振荡器核心的遗传基因。由此，我们建议进一步定义额外的昼夜节律基因在振荡器网络中的位置，并开始定义这些基因产物的生化作用模式。为此，我们将测试ELF3/ELF4复合体与光感受器和转录因子相互作用的假设，以调节时钟基因的激活；这是时钟的黄昏手臂。同时，我们将测试AKIN10对TIC的磷酸化是否会指导Coreclock蛋白的蛋白质稳定性；这是时钟的黎明之臂。这些努力通过对最近在清晨和黎明阶段进行的微阵列的分析，以及通过开发一种革命性的成像技术来描述蛋白质在活细胞内何时相互作用，在系统层面上进行了整合。这一提议的共同努力应该从根本上提高我们对植物生物钟在自然昼夜条件下运行的机械理解。