SGER: Fluorescent Detection of Bacillus anthracis

SGER：炭疽杆菌的荧光检测

• 批准号: 0204004
• 负责人: P. Buford Price
• 金额: $ 10万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0204004&HistoricalAwards=false
• 关键词: SGER; Fluorescent; Detection; Bacillus; anthracis

This Small Grant for Exploratory Research (SGER) provides funds for an examination of fluorescence as a means for identification of bacteria on solid surfaces. A number of constituents common to all organisms, including several amino acids and vitamins, fluoresce in response to ultraviolet or visible light. Because the amount, duration and wavelength of such florescence should vary with the exact composition of each organism, measurement of florescence could provide a means to identify bacteria in the presence of other materials and distinguish one type of bacteria from another. Using a synchrotron light source for irradiation of bacteria at well-defined wavelengths between 170 and 700 nm., the peak wavelength and time-decay of fluorescence by several, non-pathogenic species of Bacillus and other bacteria will be determined and then compared to the fluorescence of other particulate materials including plant pollen and clay. The goal is to investigate the potential utility of fluorescence as the basis for a device to detect bacteria on the surface of envelopes and other objects.

这项探索性研究小额赠款（SGER）为荧光检测提供资金，作为鉴定固体表面细菌的一种手段。 所有生物体共有的一些成分，包括几种氨基酸和维生素，在紫外线或可见光下会发出荧光。 由于这种荧光的量、持续时间和波长应随每种生物体的确切组成而变化，因此荧光的测量可以提供一种在存在其他材料的情况下识别细菌并将一种类型的细菌与另一种细菌区分开的方法。使用同步加速器光源在170和700 nm之间的明确定义的波长下照射细菌，将确定几种非致病性芽孢杆菌和其它细菌的荧光的峰值波长和时间衰减，然后与包括植物花粉和粘土的其它颗粒材料的荧光进行比较。 目标是研究荧光作为检测信封和其他物体表面细菌的设备的基础的潜在效用。