Mechanisms of Developmental Timing in C. Elegans

线虫的发育时间机制

• 批准号: 0212373
• 负责人: Eric Moss
• 金额: $ 36万
• 依托单位: Institute For Cancer Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0212373&HistoricalAwards=false
• 关键词: Mechanisms; Developmental; Timing; C.; Elegans

0212373MossDevelopmental timing is fundamental to all multicellular organisms. For tissues and organs to form properly, developmental events must occur in the proper succession with the appropriate synchrony. The mechanisms that explicitly govern the timing of these events are not well understood in any organism. Valuable insights, however, have come from the investigation of heterochronic genes of the nematode Caenorhabditis elegans. Dr. Moss will investigate an new and important heterochronic gene, lin-46, which helps specify the stage-appropriate fates of cells during the animal's larval development. The LIN-46 protein acts early in the developmental timing pathway, downstream of the key regulators LIN-14 and LIN-28. Proteins related to LIN-46 in plants and animals are known to mediate protein-protein interactions. Therefore, to control developmental timing LIN-46 is likely to interact with proteins that coordinate the behaviors of cells and may be regulated by LIN-14 and LIN-28.The work outlined in this proposal will address how LIN-46 governs developmental timing from two aspects. First, Dr. Moss will elucidate the molecular relationship between the lin-46 and other heterochronic genes by determining whether lin-46 expression is regulated by lin-28 and lin-14, whether they act in the same cells, and whether they are in a linear genetic pathway. Second he will characterize the interaction of LIN-46 with other proteins. He predicts that at least one other protein encoded in the C. elegans genome interacts physically with LIN-46 and is necessary for its function. He will search for other proteins that interact with LIN-46 using molecular and genetic approaches.The long-range impact of this work will be an understanding of the molecular basis of developmental timing regulation by LIN-46 and how it has evolved. What is learned about developmental timing in C. elegans may be widely applicable to other animals. The process of developmental timing is of broad biological interest because its importance in the evolution of animal form.

0212373苔藓发育的时间是所有多细胞生物的基础。为了使组织和器官正常形成，发育事件必须以适当的同步顺序发生。明确控制这些事件发生时间的机制在任何生物中都没有得到很好的理解。然而，有价值的见解，来自线虫线虫的异时基因的调查。莫斯博士将研究一种新的重要的异时基因lin-46，它有助于确定动物幼虫发育过程中细胞的阶段性命运。LIN-46蛋白在发育时序途径的早期起作用，位于关键调节因子LIN-14和LIN-28的下游。已知植物和动物中与LIN-46相关的蛋白质介导蛋白质-蛋白质相互作用。因此，LIN-46可能与细胞行为协调蛋白相互作用，并受LIN-14和LIN-28的调控，从而调控发育时序。首先，Moss博士将通过确定lin-46的表达是否受lin-28和lin-14的调节，它们是否在相同的细胞中起作用，以及它们是否处于线性遗传途径，来阐明lin-46和其他异时基因之间的分子关系。其次，他将描述LIN-46与其他蛋白质的相互作用。他预测，至少有一种其他蛋白质编码的C。线虫基因组与LIN-46物理相互作用，并且是其功能所必需的。他将使用分子和遗传学方法寻找与LIN-46相互作用的其他蛋白质。这项工作的长期影响将是对LIN-46发育时序调节的分子基础及其进化方式的理解。在C语言中学习到的关于发展时间的知识。秀丽隐杆线虫可能广泛适用于其他动物。发育时间的过程是广泛的生物学兴趣，因为它在动物形态的进化中的重要性。