Developmental timing mechanisms during the formation of the compound eye in the honeybee worker, queen and drone (A07 [B03]+)

工蜂、蜂王和雄蜂复眼形成过程中的发育计时机制 (A07 [B03] )

• 批准号: 234402740
• 金额: --
• 依托单位: Lehrstuhl für Verhaltensphysiologie und Soziobiologie (Zoologie II)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/234402740?language=en
• 关键词: Developmental; timing; mechanisms; during; formation

The project aims to identify and characterize the light input pathways of the circadian system in the bumblebee, Bombus terrestris. We will measure daily activity rhythms of bumblebees in a locomotor activity monitor under different monochromatic light regimes to determine the relative contribution of specific photoreceptors, and the two major visual systems of the bee, the compound eyes and the ocelli. Since bumblebees lack the blue light-sensitive flavoprotein Cryptochrome (CRY1), we will test the hypothesis that Pteropsin, a putative nonvisual brain photopigment, replaces some functions of Cryptochrome. In a joint collaboration with various groups within the CRC, we also aim to establish the CRISPR/Cas9 system for non-model animals, such as bees and ants, to knockout specific photoreceptors and subsequently test such animals for light entrainment.

该项目旨在确定和表征熊蜂昼夜节律系统的光输入途径。我们将在不同的单色光制度下测量大黄蜂的日常活动节律，以确定特定的光感受器的相对贡献，以及蜜蜂的两个主要视觉系统，复眼和单眼。由于熊蜂缺乏蓝光敏感的黄素蛋白隐花色素（Cryptochrome，简称Pteropsin），我们将测试这一假设，即Pteropsin，一种假定的非视觉脑色素，取代了隐花色素的一些功能。在与CRC内的各个小组的联合合作中，我们还旨在为非模型动物（如蜜蜂和蚂蚁）建立CRISPR/Cas9系统，以敲除特定的光感受器，并随后测试这些动物的光夹带。