SBIR Phase I: New Beta-Glucurondise (GUS) Substrates for Cell Regulation

这个小企业创新研究（SBIR）第一阶段项目是开发商业用途的结合物，能够利用克隆的基因表达在转化的植物细胞中来控制它们的生长和生理。本项目的具体目标是合成一系列新的植物调节物质的β -葡糖醛酸缀合物，并测试这些缀合物对表达β -葡糖醛酸酶（GUS）标记基因的特定组织和细胞的生长和蛋白质表达的影响。结合物将在组织培养和整株植物中进行测定，以确定是否能以依赖启动子的方式引起局部细胞死亡。这些化合物将广泛用于植物发育和基因表达的控制研究，并有可能通过选择性应用底物和选择报告基因和启动子元件，以可逆的方式产生农业上重要的植物物种。该项目的商业应用是在农业生物技术领域。

This Small Business Innovation Research (SBIR) Phase I project is to develop commercial uses for conjugates capable of exploiting cloned gene expression in transformed plant cells to control their growth and physiology. The specific goal of this project is to synthesize a series of new beta-glucuronide conjugates of plant regulatory substances and to test these conjugates for their ability to modify the growth and protein expression of specific tissues and cells expressing the beta-glucuronidase (GUS) marker gene. Conjugates will be assayed in tissue culture and in whole plants for the ability to cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research on the control of development and gene expression and have the potential to produce agriculturally important plant species in a reversible manner, through the use of selective application of substrate and choice of reporter gene and promoter elements.The commercial application of this project is in the area of agricultural biotechnology.