Glucuronide Plant Regulation

利用大肠杆菌β -葡萄糖醛酸酶基因（GUS）作为融合标记分析转化植物中的基因表达已经得到证实（Jefferson等，1987年）。采用化学和酶法合成了一系列植物调节物质的葡萄糖醛酸缀合物。在第一阶段，我们将合成除草剂和毒素的偶联物，并测试这些偶联物特异性消融表达GUS活性的组织和细胞的能力。结合物将在组织培养和整株植物中进行试验，以确定其以启动子依赖的方式引起局部细胞死亡的能力。这些化合物将在植物研究中广泛应用于发育和基因表达的控制，并有可能通过选择性应用底物和选择gus融合启动子，以可逆的方式产生农业上重要的不育植物物种。

Use of the Escherichia coli Beta-glucuronidase gene (GUS) as a fusion marker for analysis of gene expression in transform plants has been demonstrated (Jefferson, et al. 1987). The synthesis, by chemical and enzymatic methods, of a series of glucuronic acid conjugates of plant regulatory substances is proposed. In Phase I, we will be we will synthesize conjugates of herbicides and toxins and test these conjugates for the ability to specifically ablate tissues and cells expressing GUS activity. Conjugates will be assayed in tissue culture and in whole plants for the ability top cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research into the control of development and gene expression and have the potential to produce agriculturally important sterile plant species in a reversible manner, through the use of selective application of substrate and choice of GUS-fusion promoter.