SBIR Phase I: New Labeling Reagents for Genetic Analysis

SBIR 第一阶段：用于遗传分析的新型标记试剂

• 批准号: 0740717
• 负责人: John Naleway
• 金额: --
• 依托单位: MARKER GENE TECHNOLOGIES, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0740717&HistoricalAwards=false
• 关键词: SBIR; Phase; New; Labeling; Reagents

This Small Business Innovation Research (SBIR) Phase I research project aims to develop novel nucleic acid labeling reagents that can be used in living cells to monitor gene expression. Instead of relying on labeling the molecules using fluorescently labeled bases, the proposed approach relies on adding the label to the terminal phosphates. This would have great advantage over existing methods and would be a welcome addition to the tools of molecular analysis.Current detection of the changes in gene expression relies on the labeling of nucleic acids with fluorescent labels, which sometimes interferes with hybridization. Thus development of a technology that allows labeling of the nucleic acids in such a way so as to not affect the molecules? hybridization parameters would be a significant advance. Moreover, such a technology would make quantitiation of the hybridization event easier as the fluorescence intensity of the probe molecules would not be sequence dependent. In addition, the obviation of the need for enzyme incorporation and amplification methods would reduce the time and cost associated with the labeling of probes.

这个小型企业创新研究（SBIR）第一阶段研究项目旨在开发可用于活细胞监测基因表达的新型核酸标记试剂。 所提出的方法不依赖于使用荧光标记的碱基标记分子，而是依赖于将标记添加到末端磷酸盐。 这将比现有的方法有很大的优势，并将是一个受欢迎的除了分子分析的工具。目前的检测基因表达的变化依赖于标记的核酸与荧光标记，这有时会干扰杂交。 因此，开发一种技术，允许标记的核酸在这样一种方式，以便不影响分子？杂交参数将是一个显著的进步。 此外，这种技术将使杂交事件的定量更容易，因为探针分子的荧光强度将不是序列依赖性的。 此外，不需要酶掺入和扩增方法将减少与探针标记相关的时间和成本。