SBIR Phase I: Luciferase Directed Substrates for Cell Regulation

SBIR 第一阶段：用于细胞调节的荧光素酶导向底物

• 批准号: 9960378
• 负责人: John Naleway
• 金额: $ 9.95万
• 依托单位: MARKER GENE TECHNOLOGIES, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9960378&HistoricalAwards=false
• 关键词: SBIR; Phase; Luciferase; Directed; Substrates

This Small Business Innovation Research Phase I project aims to develop commercial uses for conjugates capable of exploiting firefly luciferase expression in transformed plant cells to control their growth and physiology. The synthesis of a series of D-luciferin conjugates of plant regulatory substances is researched. In Phase I, we will test these conjugates for the ability to ablate specific tissues and cells expressing luciferase (luc) activity. Conjugates will be assayed in tissue culture and in whole plants for the ability to cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research on the control of development and gene expression and have the potential to produce agriculturally important sterile plant species in a reversible manner, through the use of selective application of substrate and choice of luc-fusion promoter.

这个小企业创新研究第一阶段项目旨在开发能够利用转化植物细胞中萤火虫荧光素酶表达的缀合物的商业用途，以控制其生长和生理学。研究了一系列植物调节物质D-精氨酸偶联物的合成。在I期，我们将测试这些缀合物消融表达荧光素酶（luc）活性的特定组织和细胞的能力。将在组织培养物和整个植物中测定缀合物以启动子依赖性方式引起局部细胞死亡的能力。 此类化合物将普遍用于控制发育和基因表达的植物研究，并且具有通过使用底物的选择性施用和luc-fusion启动子的选择以可逆方式产生农业上重要的不育植物物种的潜力。