Acquisition of Instrumentation for Proteome Analysis Core

购置蛋白质组分析核心仪器

• 批准号: 0215759
• 负责人: David Bernlohr
• 金额: $ 49.09万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0215759&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Proteome; Analysis; Core

A grant has been awarded to the University of Minnesota-St. Paul, under the supervision of Dr. David A. Bernlohr to purchase equipment and technology in a dedicated facility termed the proteome analysis core. The resources allocated to the proteome analysis core will be used to develop a core facility for rapid, quantitative, high-volume detection of proteins from plants, animals, insects and bacteria. The rapid detection and identification of large numbers of specific proteins from mixtures and extracts is technically difficult, but is essential for a variety of biological applications including bio-identification, evaluation of treatment or conditions (bio-responses) and assessment of development (crop growth). The first goal of the project is to provide researchers with the experimental technology necessary to assess expression of fluorescently labeled protein samples using nano-scale separation methodologies. The second component of the technology is the utilization of a robotically driven sample analysis workstation that will interface with an existing mass spectrometry facility. The combination of fluorescence-based protein detection coupled with rapid sample identification via mass spectrometry allows the user accurate identification of large numbers of proteins for a variety of basic and translational research needs. Specifically, the proteome analysis core will instruct researchers on the use of differential in-gel electrophoresis for the analysis of fluorescently labeled proteins. The researcher will in turn be able to identify changes in protein expression or protein modification (precursor-product relationship). Using a robotically driven analysis system, target proteins will be harvested from the gel, enzymatically digested, and the produced peptides spotted onto a MALDI plate. The peptides are subsequently analyzed via mass spectrometry and using genome databases, identify proteins of interest. The short time of analysis coupled with robotically driven workstation allows for scale-up and the analysis of hundreds of samples of proteins. The major theme of the proteome analysis core is one of training and education. As opposed to an analytical service facility, the proteome analysis core trains students, postdocs, and faculty such that they themselves can manage a project. As such, the technology has broad significance for training students in such diverse research areas such as crop and animal science, horticulture, bioremediation, and environmental science. Because the proteome analysis core will be physically located in the BioDale technology corridor on the St. Paul campus of the University of Minnesota, it will be adjacent to other facilities linked to Genome Analysis, Imaging, Mass Spectrometry and Bioprocess Technology. Course work, both during the academic year and during summer session, are being planned to train students in proteomics and mass spectrometry. During the summer, minority scholars participating in the Life Sciences Undergraduate Research Program will make use of the proteome analysis core through workshops and their own research projects. The availability of such a training site will allow the Univesity of Minnesota to attract, train, and recruit underrepresented minorities into our undergraduate and graduate programs and ultimately into science and technology based careers.

明尼苏达大学圣路易斯分校获得了一笔资助。 Paul 在 David A. Bernlohr 博士的监督下购买了称为蛋白质组分析核心的专用设施中的设备和技术。 分配给蛋白质组分析核心的资源将用于开发一个核心设施，用于快速、定量、大量检测来自植物、动物、昆虫和细菌的蛋白质。 从混合物和提取物中快速检测和鉴定大量特定蛋白质在技术上很困难，但对于各种生物应用至关重要，包括生物鉴定、处理或条件评估（生物反应）和发育评估（作物生长）。 该项目的首要目标是为研究人员提供使用纳米级分离方法评估荧光标记蛋白质样品表达所需的实验技术。 该技术的第二个组成部分是利用机器人驱动的样品分析工作站，该工作站将与现有的质谱设施连接。 基于荧光的蛋白质检测与通过质谱进行快速样品鉴定相结合，使用户能够准确鉴定大量蛋白质，以满足各种基础和转化研究的需求。 具体来说，蛋白质组分析核心将指导研究人员使用差异凝胶内电泳来分析荧光标记的蛋白质。 研究人员进而能够识别蛋白质表达或蛋白质修饰（前体-产物关系）的变化。 使用机器人驱动的分析系统，将从凝胶中收获目标蛋白，进行酶消化，并将产生的肽点样到 MALDI 板上。 随后通过质谱法和基因组数据库对肽进行分析，识别感兴趣的蛋白质。 分析时间短，加上机器人驱动的工作站，可以扩大规模并分析数百个蛋白质样品。 蛋白质组分析核心的主题之一是培训和教育。 与分析服务设施不同，蛋白质组分析核心培训学生、博士后和教师，使他们自己能够管理项目。 因此，该技术对于培养作物和动物科学、园艺、生物修复和环境科学等不同研究领域的学生具有广泛的意义。 由于蛋白质组分析核心物理位置将位于明尼苏达大学圣保罗校区的 BioDale 技术走廊内，因此它将毗邻与基因组分析、成像、质谱和生物工艺技术相关的其他设施。 计划在学年和夏季期间开设课程，以培训学生蛋白质组学和质谱学方面的知识。 夏季期间，参与生命科学本科生研究计划的少数族裔学者将通过研讨会和自己的研究项目利用蛋白质组分析核心。 这样一个培训网站的可用性将使明尼苏达大学能够吸引、培训和招募代表性不足的少数族裔进入我们的本科和研究生课程，并最终进入以科学和技术为基础的职业。