MRI: Acquisition of Microscopy Instrumentation for Multi-Disciplinary Research and Research Training

摘要：一项研究经费已被授予博士。John T. Longino, Elizabeth M. Kutter和Erik V. Thuesen在Evergreen State College获得了一台变压扫描电子显微镜，一台倒置复合荧光显微镜和一台立体显微镜，包括电动驱动器和允许通过互联网远程操作的计算机接口。该仪器将用于研究热带生物多样性、生物膜中的微生物过程和海洋浮游动物的生态生理学。约翰·朗吉诺的研究主要集中在这里和哥斯达黎加的蚂蚁身上。可变压力扫描电子显微镜和立体显微镜设备将用于完成哥斯达黎加火化蚁的分类修订，这是树栖蚂蚁的主要属之一。产品将是传统的出版作品和网络上的图像丰富的识别指南。Elizabeth Kutter将使用荧光显微镜来研究生物膜中细菌的噬菌体感染——生物膜是一种非常广泛、复杂的螺旋塔和流体通道群落，它们对抗生素和消毒剂有很大的抗药性。埃里克·图森正在研究胶状浮游动物在低氧环境下的生存能力。他假设了一种便利的扩散机制，将使用荧光显微镜检查线粒体的分布进行研究。常青大学的科学教学以研究为主。许多常青学院的教职员工在开展研究和教学项目时，很大程度上依赖于显微镜，这些仪器将大大增强他们拓展和向新方向发展的能力。除了对私家侦探的显微镜研究许多其他学院让学生积极参与当地资助的小规模研究项目和报告，其中许多涉及复杂的显微镜。这包括对有害藻华、环境微生物学和土壤生物学的研究。除了这些特定的研究活动之外，这些仪器还将用于其他方面，通过接触令人兴奋的图像和概念，吸引广泛的学生，并改善代表性不足的学生群体的机会，特别是在我们的塔科马校区，该校区为高比例的内城学生提供服务。远程控制能力将支持已经在塔科马校区启动的生物修复和微生物学方面的严肃研究项目，并将显微镜的使用从实验室扩展到教室。常青学院的教学本身也是跨学科的，扫描电镜和其他显微镜将成为艺术和科学综合全日制课程的重点。

AbstractA grant has been awarded to Drs. John T. Longino, Elizabeth M. Kutter, and Erik V. Thuesen at The Evergreen State College to acquire a variable-pressure scanning electron microscope, an inverted compound fluorescent microscope, and a stereomicroscope, including motorized drives and computer interfaces that allow remote operation over the internet. The Instrumentation will be used In studies of tropical biodiversity, microbial processes in biofilms, and ecophysiology of marine zooplankton. John Longino's research focuses primarily on ants, here and in Costa Rica. The variable-pressure scanning electron microscope and stereomicroscope equipment will be used to finish a taxonomic revision of the Costa Rican Crematogaster, one of the major genera of arboreal ants. The products will be both traditional published work and image-rich identification guides on the web. Elizabeth Kutter will use the fluorescent microscope to study phage infections of bacteria in biofilms - very widespread, elaborate communities of convoluted towers and fluid channels that are largely resistant to antibiotics and disinfectants. Erik Thuesen is investigating the ability of gelatinous zooplankton to survive in low-oxygen environments. He hypothesizes a facilitated diffusion mechanism which will be investigated using fluorescent microscopy to examine the distribution of mitochondria. Science teaching at Evergreen is strongly research oriented. Many Evergreen faculty members carry out research and teaching programs that rely substantially on microscopy, and their ability to expand and move in new directions will be greatly enhanced by these instruments. In addition to the microscopy-intensive research of the P.I.'s, a number of other faculty involve their students actively in smaller-scale, locally-funded research projects and presentations, many of which involve sophisticated microscopy. This includes research into harmful algal blooms, environmental microbiology and soil biology. The instrumentation will also be used in ways beyond these specific research activities, attracting a broad range of students through exposure to exciting images and concepts and improving access for underrepresented groups of students, especially at our Tacoma campus, which serves a high proportion of inner city students. The remote control capabilities will support serious research projects in bioremediation and microbiology already initiated at the Tacoma campus and expand microscope use from laboratory to classroom. Teaching at Evergreen is also inherently and intentionally interdisciplinary, and the SEM and other microscopes will serve as foci for full-time programs integrating arts and sciences.