MRI: Acquisition of a Zeiss LSM980 Laser Scanning Confocal Microscope with Airyscan 2 for Microscopy Core Facility

MRI：采购蔡司 LSM980 激光扫描共焦显微镜和 Airyscan 2 用于显微镜核心设施

• 批准号: 2117484
• 负责人: Edward Bonder
• 金额: $ 55.45万
• 依托单位: Rutgers University Newark
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117484&HistoricalAwards=false
• 关键词: MRI; Acquisition; Zeiss; LSM980; Laser

An award is made to Rutgers University – Newark to acquire a Zeiss LSM980 Laser Scanning Confocal Microscope with Airyscan 2. The Zeiss LSM 980 will be integrated into the Advanced Imaging Core Facility (AICF), of the Department of Biological Sciences (DBS), a core university facility that serves Rutgers and institutions of higher education in Newark. Installation of the microscope provides critically needed instrumentation that will have an unquestionable positive impact at Rutgers-Newark as an anchor institution with broad mission to improve and advance STEM education, training, and outreach. Acquisition of the Zeiss LSM 980 will initiate improvement in the undergraduate curriculum in Biology by stimulating development of a six-week long biological imaging laboratory series in a core biology course that enrolls 600 students annually. Using in-person and virtual teaching/learning strategies, students will be able to have an exhilarating first-hand experience at multi-dimensional imaging. DBS will add hands-on laser confocal imaging to a capstone upper division laboratory course in microscopy. To reach a broader undergraduate and graduate student audience, the leadership team will work with NSF-LSAMP, NIH-G-RISE, and Rutgers-NASA Space Grant Consortium programs at Rutgers-Newark to introduce multi-dimensional imaging using the Zeiss LSM 980. It is anticipated that this approach will encourage increased undergraduate participation in research, particularly URM students, and encourage pursuit of STEM careers. STEM faculty at Rutgers-Newark have a long-standing tradition of partnering with American Chemical Society Project SEED and Newark Public Schools to provide urban high school students with hands-on laboratory experience. Microscopy is often a key part of that experience. The leadership team is invested in bringing the wonder of fluorescence imaging to local high school students through the 4-week summer immersion program RUN-IMAGE and the academic year program Aim High NPS-NorthStar Academy. Each of these programs will benefit from both hands-on and virtual imaging relying upon the Zeiss LSM 980 and other microscopes in the AICF. The addition of the Zeiss LSM 980 to the AICF will have societal impacts on multiple fronts. Introducing advanced imaging to students at all levels will improve science literacy and build an understanding of the process of scientific discovery and application. This will have a future positive impact as US citizens are asked to make important societal decisions dependent upon an understanding of science. Further, the Zeiss LSM 980 will strengthen the ability of Rutgers-Newark to hire and retain top-shelf faculty whose research and teaching skills will add to education, training, and outreach at Rutgers-Newark. The LSM 980 will provide new faculty in the biological science with an essential modern-day research instrument that is vital for success in obtaining extramural grant funding and in publishing peer-reviewed work.The Zeiss LSM 980 with Airyscan 2 provides a state-of-the-art microscopy workstation that provides multi-dimensional fluorescence imaging as well as super-resolution research capacity. The microscope will support fundamental research examining the mechanisms of cell signaling and membrane-protein sorting in neural, intestinal epithelial, and bacterial cells. The Zeiss LSM 980 will enable researchers will use novel fluorescent-reporter proteins in conjunction with innovative genetic animal and in vitro cell/organoid models to follow the dynamics and regulatory function of small GTPases and transcription factors in regulating cell and tissue differentiation and maintenance. Super-resolution fluorescence imaging and photo-manipulation will be used to discover novel protein-membrane and protein-protein interactions in the assembly of macromolecular complexes that regulate lipid transport, assembly of cell surface receptors, and intracellular sorting of signaling complexes in bacterial membranes, during myelin formation in peripheral nervous system, and embryonic development in mammalian central. The Zeiss LSM980 will be used in state-of-the-art optogenetic experimentation on the important role of cytoskeleton-chromatin interactions on gene expression and epigenetics. Additionally, the microscope will be used to provide multi-dimensional fluorescence imaging of cell signaling and remodeling pathways activated in response to tissue and cell injury from trauma including, impact, stretch, radiation, and chemical insult. All the research projects are fully integrated with STEM education/training of high school, undergraduate, graduate students and postdoctoral students serving as the foundation for improved STEM literacy and future leadership.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予罗格斯大学-纽瓦克分校一项合同，购买配备Airyscan 2的蔡司LSM 980激光扫描共聚焦显微镜。 蔡司LSM 980将被集成到生物科学系（DBS）的高级成像核心设施（AICF）中，这是一个为罗格斯大学和纽瓦克高等教育机构提供服务的核心大学设施。 显微镜的安装提供了急需的仪器，这将在罗格斯-纽瓦克作为一个锚机构具有广泛的使命，以改善和推进干教育，培训和推广产生毫无疑问的积极影响。 收购蔡司LSM 980将启动生物学本科课程的改进，刺激开发为期六周的生物成像实验室系列，每年招收600名学生。 使用面对面和虚拟教学/学习策略，学生将能够在多维成像方面获得令人兴奋的第一手经验。 DBS将增加动手激光共聚焦成像的顶点上师实验室课程显微镜。 为了覆盖更广泛的本科生和研究生受众，领导团队将与NSF-LSAMP，NIH-G-RISE和Rutgers-NASA太空资助联盟计划合作，在Rutgers-Newark推出使用蔡司LSM 980的多维成像。 预计这种方法将鼓励更多的本科生参与研究，特别是URM学生，并鼓励追求STEM职业。罗格斯大学纽瓦克分校的STEM教师长期以来一直与美国化学学会项目SEED和纽瓦克公立学校合作，为城市高中学生提供动手实验室经验。 显微镜通常是这种体验的关键部分。领导团队致力于通过为期4周的暑期沉浸式课程RUN-IMAGE和学年计划Aim High NPS-NorthStar Academy将荧光成像的奇迹带给当地高中生。 这些项目中的每一个都将受益于蔡司LSM 980和AICF中其他显微镜的实际操作和虚拟成像。 蔡司LSM 980加入AICF将在多个方面产生社会影响。 向各级学生介绍先进的成像技术将提高科学素养，并建立对科学发现和应用过程的理解。 这将对未来产生积极影响，因为美国公民被要求根据对科学的理解做出重要的社会决策。 此外，蔡司LSM 980将加强罗格斯-纽瓦克大学聘用和留住顶级教师的能力，这些教师的研究和教学技能将增加罗格斯-纽瓦克大学的教育、培训和推广。 LSM 980将为生物科学领域的新教师提供一个必不可少的现代研究仪器，这对于成功获得校外资助和发表同行评审工作至关重要。配备Airyscan 2的蔡司LSM 980提供了一个最先进的显微镜工作站，可提供多维荧光成像以及超分辨率研究能力。 该显微镜将支持基础研究，检查神经，肠上皮和细菌细胞中细胞信号传导和膜蛋白分选的机制。 Zeiss LSM 980将使研究人员能够将新型荧光报告蛋白与创新的遗传动物和体外细胞/类器官模型结合使用，以跟踪小GTP酶和转录因子在调节细胞和组织分化和维持中的动力学和调节功能。 超分辨率荧光成像和光操纵将用于发现新的蛋白质-膜和蛋白质-蛋白质相互作用的大分子复合物的组装，调节脂质转运，细胞表面受体的组装，以及细菌膜中信号复合物的细胞内分选，在周围神经系统的髓鞘形成期间，以及哺乳动物中枢的胚胎发育。 蔡司LSM 980将用于最先进的光遗传学实验，研究细胞色素-染色质相互作用对基因表达和表观遗传学的重要作用。 此外，显微镜将用于提供细胞信号传导和重塑通路的多维荧光成像，这些通路响应于创伤（包括冲击、拉伸、辐射和化学损伤）引起的组织和细胞损伤而激活。 所有的研究项目都与高中、本科、研究生和博士后的STEM教育/培训完全结合，作为提高STEM素养和未来领导力的基础。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。