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Molecular mechanism of high force generation by bacterial pili

细菌菌毛产生高力的分子机制

基本信息

批准号：
158987927
负责人：
Professorin Dr. Berenike Maier
金额：
--
依托单位：
Institut für biologische Physik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2020-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/158987927?language=en
关键词：
Molecular mechanism force generation bacterial

项目摘要

The development of bacterial biofilms resembles eukaryotic development in various aspects. It was shown that mechanical forces are involved in establishing the tissue architecture of embryos. However, little is known about the role of mechanical forces during biofilm formation. In this project, we will use the type IV pilus (T4P) of Neisseria gonorrhoeae as a tool for controlling force generation between bacteria. During the first project phase we generated mutants that generate different levels of force by T4P retraction. Here, we will use these mutants to address the question how the cell-to-cell interaction forces influence the biofilm local microstructure including the radial distribution function. The influence of interaction forces on the rheological properties of biofilm will be characterized as a function of biofilm age. Moreover, we hypothesize that cell-cell contact and biofilm age affect force generation by bacteria. This hypothesis will be scrutinized by characterizing the intracellular localization and binding kinetics of the pilus retraction ATPase PilT and its antagonist PilF using PALM and single molecule tracking. It is tempting to speculate that the local biofilm structure affects the accessibility of nutrients, oxygen, and antimicrobials. As a long term goal, we will assess how the cell-to-cell interaction force affects the fitness of bacteria within the biofilm under benign conditions and under external stress.

细菌生物膜的发育在许多方面类似于真核生物的发育。结果表明，机械力参与建立胚胎的组织结构。然而，鲜为人知的是，在生物膜形成过程中的机械力的作用。在这个项目中，我们将使用淋球菌的IV型菌毛（T4 P）作为控制细菌之间产生力的工具。在第一个项目阶段，我们产生了突变体，通过T4 P收缩产生不同水平的力。在这里，我们将使用这些突变体来解决的问题，如何细胞间的相互作用力影响生物膜局部微观结构，包括径向分布函数。相互作用力对生物膜流变特性的影响将被表征为生物膜年龄的函数。此外，我们假设细胞-细胞接触和生物膜年龄影响细菌的力产生。将通过使用PALM和单分子追踪表征菌毛收缩ATP酶PilT及其拮抗剂PilF的细胞内定位和结合动力学来仔细检查该假设。很容易推测，局部生物膜结构影响营养物质、氧气和抗菌剂的可及性。作为一个长期的目标，我们将评估细胞间的相互作用力如何影响在良性条件下和外部压力下生物膜内细菌的适应性。