ME: Collaborative Research: Deployment of Enhanced Stress Responses to Improve Recombinant Expression Systems
ME:协作研究:部署增强的应激反应以改进重组表达系统
基本信息
- 批准号:0224733
- 负责人:
- 金额:$ 36.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-09-01 至 2006-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The objective of this project is to improve recombinant expression systems. The major goals are to discover and characterize pathways that facilitate protein folding and subunit assembly in hyperthermophiles. The components of these pathways will be inserted into recombinant host cells to preempt induction of stress responses during recombinant expression, and to enhance the stabilization and solubilization of recombinant protein. Building on the approach and findings of the investigators previous research, the specific objectives are: (1) to determine the functions of interacting proteins encoded by the 23 genes in the heat shock regulon of the hyperthermophile, Pyrococcus furiosus, (2) to identify functional complexes of thermostable heat shock proteins and express them in E. coli, yeast and mammalian cells to enhance the durability of these cells, and (3) to determine the effects of combinations of chaperonins on high level recombinant expression of proteins in intact cells as well as cell-free transcription-translation systems.
本项目的目的是改进重组表达系统。 主要目标是发现和表征促进超嗜热菌中蛋白质折叠和亚基组装的途径。 将这些途径的组分插入重组宿主细胞中,以在重组表达期间抢先诱导应激反应,并增强重组蛋白的稳定性和溶解性。 本论文在前人研究的基础上,具体目标是:(1)确定热激调节子中23个基因编码的相互作用蛋白的功能;(2)鉴定热稳定热激蛋白的功能复合物,并在大肠杆菌中表达。大肠杆菌、酵母和哺乳动物细胞,以增强这些细胞的耐久性,以及(3)确定伴侣蛋白的组合对完整细胞以及无细胞转录-翻译系统中蛋白质的高水平重组表达的影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Douglas Clark其他文献
Structural Changes in the Forest Sector and Their Long-term Consequences for the Forest Sector
林业部门的结构变化及其对林业部门的长期影响
- DOI:
10.18356/9789210023719 - 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Douglas Clark - 通讯作者:
Douglas Clark
Pharmacokinetics and Pharmacodynamics of CD4-Anchoring Bi-Functional Fusion Inhibitor in Monkeys
CD4锚定双功能融合抑制剂在猴体内的药代动力学和药效学
- DOI:
10.1007/s11095-013-1203-4 - 发表时间:
2014 - 期刊:
- 影响因子:3.7
- 作者:
Xingrong Liu;Y. Ou;Jun Zhang;A. Ahene;Douglas Clark;S. Hsieh;M. Cooper;Changhua Ji - 通讯作者:
Changhua Ji
Inhaled Corticosteroid Use and Risk of Non-vertebral Fracture Among Adults With Chronic Obstructive Lung Disease in UK General Practice
- DOI:
10.1378/chest.124.4_meetingabstracts.166s-a - 发表时间:
2003-01-01 - 期刊:
- 影响因子:
- 作者:
Kourtney J. Davis;Douglas Clark;Katherine Knobil - 通讯作者:
Katherine Knobil
Clark, Timothy W., Murray B. Rutherford, and Denise Casey (eds): Coexisting with Large Carnivores: Lessons from Greater Yellowstone
- DOI:
10.1007/s11077-007-9037-8 - 发表时间:
2007-09-06 - 期刊:
- 影响因子:3.700
- 作者:
Douglas Clark - 通讯作者:
Douglas Clark
Douglas Clark的其他文献
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{{ truncateString('Douglas Clark', 18)}}的其他基金
EAGER: Testing Late-Pleistocene and Holocene Climate Change in Southeastern Australia with Multi-proxy Analyses of Alpine Lake Sediments, Kosciuszko National Park, Australia
EAGER:通过对澳大利亚科修斯科国家公园高山湖泊沉积物的多代理分析来测试澳大利亚东南部更新世晚期和全新世气候变化
- 批准号:
2023027 - 财政年份:2020
- 资助金额:
$ 36.59万 - 项目类别:
Standard Grant
Quinquennial (half-decadal) carbon and nutrient dynamics in temperate forests: Implications for carbon sequestration in a high carbon dioxide world
温带森林五年(半十年)碳和养分动态:对高二氧化碳世界中碳封存的影响
- 批准号:
NE/S015744/2 - 财政年份:2019
- 资助金额:
$ 36.59万 - 项目类别:
Research Grant
Quinquennial (half-decadal) carbon and nutrient dynamics in temperate forests: Implications for carbon sequestration in a high carbon dioxide world
温带森林五年(半十年)碳和养分动态:对高二氧化碳世界中碳封存的影响
- 批准号:
NE/S015744/1 - 财政年份:2019
- 资助金额:
$ 36.59万 - 项目类别:
Research Grant
The UK Earth system modelling project.
英国地球系统建模项目。
- 批准号:
NE/N017951/1 - 财政年份:2016
- 资助金额:
$ 36.59万 - 项目类别:
Research Grant
Enhancing Games with Assessment and Metacognitive Emphases (EGAME)
通过评估和元认知重点增强游戏 (EGAME)
- 批准号:
1119290 - 财政年份:2011
- 资助金额:
$ 36.59万 - 项目类别:
Continuing Grant
E: Scaffolding Understanding by Redesigning Games for Education (SURGE)
E:通过重新设计教育游戏来理解脚手架(SURGE)
- 批准号:
1019170 - 财政年份:2010
- 资助金额:
$ 36.59万 - 项目类别:
Standard Grant
Collaborative Research: P2C2--Ice Core Paleoclimate Records from Combatant Col, British Columbia, Canada
合作研究:P2C2——加拿大不列颠哥伦比亚省战斗上校的冰芯古气候记录
- 批准号:
0902392 - 财政年份:2009
- 资助金额:
$ 36.59万 - 项目类别:
Standard Grant
E: Scaffolding Understanding by Redesigning Games for Education (SURGE)
E:通过重新设计教育游戏来理解脚手架(SURGE)
- 批准号:
0822370 - 财政年份:2008
- 资助金额:
$ 36.59万 - 项目类别:
Standard Grant
Collaborative Research: SGER: Ice Core Paleoclimate Record from Mt. Waddington, British Columbia Coast Range
合作研究:SGER:来自不列颠哥伦比亚省海岸山脉沃丁顿山的冰芯古气候记录
- 批准号:
0629497 - 财政年份:2006
- 资助金额:
$ 36.59万 - 项目类别:
Standard Grant
Biocatalyst Engineering for Maximum Activity in Nonaqueous Media
在非水介质中实现最大活性的生物催化剂工程
- 批准号:
0228145 - 财政年份:2003
- 资助金额:
$ 36.59万 - 项目类别:
Continuing Grant
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Data Management and Coordinating Center (DMCC) for the Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome (ME/CFS) Collaborative Research Centers (CRC)
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Canadian Collaborative Team Conference on ME/CFS: "Advancing an International Research Agenda to Address ME/CFS Research Priorities"
加拿大 ME/CFS 合作团队会议:“推进国际研究议程以解决 ME/CFS 研究优先事项”
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373947 - 财政年份:2017
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Miscellaneous Programs
Cornell ME/CFS Collaborative Research Center Administrative Core
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10627288 - 财政年份:2017
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