AWARE-Japan: The Role of Akt/PKB in the Maturation of Marine Worm Eggs

AWARE-Japan：Akt/PKB 在海洋蠕虫卵成熟中的作用

• 批准号: 0225819
• 负责人: Stephen Stricker
• 金额: $ 0.56万
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-15 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0225819&HistoricalAwards=false
• 关键词: AWARE; Japan; Role; Akt; PKB

0225819StrickerThis award supports a one-year collaborative research project between Professor Stephen Stricker of the University of New Mexico and Professor Takeo Kishimoto of the Tokyo Institute of Biosciences and Biotechnology. The researchers will undertake a study on the role of Akt PKB in the maturation of marine worm eggs. For fertilization and development to proceed normally, eggs characteristically must undergo a maturation process that is mediated by an important cell cycle modulator, termed maturation-promoting factor (MPF). Immature eggs (="oocytes") of nemerteans spontaneously mature after contacting natural seawater (NSW) by undergoing a process called "germinal vesicle breakdown" (GVBD). Such GVBD is blocked by isolating oocytes in calcium-free SW (CaFSW), but this inhibition can be overridden by adding low doses of the neurohormone serotonin (=5-HT). The researchers will attempt to determine if during 5-HT-induced GVBD in nemertean oocytes, Akt/PKB is activated in a MAPK-and cdc25-independent manner that in turn downregulates Myt-1 and thereby activates MPF as in starfish oocytes. The research will be centered on optical imaging and spectroscopic techniques with high spatial and temporal resolution. The project brings together the efforts of two laboratories that have complementary expertise and research capabilities. Professor Kishimoto's laboratory in Japan is one of the leading centers in the world for investigations of MPF regulation in a variety of cells, including maturing eggs. The lab specializes in studies of starfish egg maturation. The lab practices a low-pressure form of microinjection that is particularly well suited for quantitative injections required for antibody inactivations of Akt. Professor Stricker's laboratory utilizes an alternative high-pressure microinjection system that does not readily lend itself to quantitation. The research will offer a good opportunity to join efforts between the two countries. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation. The project advances international human resources through the participation of a graduate student

0225819 Stricker该奖项支持新墨西哥州大学Stephen Stricker教授和东京生物科学与生物技术研究所Takeo Kishimoto教授之间为期一年的合作研究项目。 研究人员将对Akt PKB在海洋蠕虫卵成熟中的作用进行研究。 为了使受精和发育正常进行，卵子必须经历一个成熟过程，该过程由一种重要的细胞周期调节剂介导，称为成熟促进因子（MPF）。 纽形动物的未成熟卵（=“卵母细胞”）在接触天然海水（NSW）后通过经历称为“生殖囊泡破裂”（GVBD）的过程自发成熟。 这种GVBD通过将卵母细胞分离在无钙SW（CaFSW）中来阻断，但这种抑制可以通过添加低剂量的神经激素5-羟色胺（=5-HT）来克服。 研究人员将试图确定在纽虫卵母细胞中5-HT诱导的GVBD期间，Akt/PKB是否以不依赖于MAPK和cdc 25的方式被激活，从而下调Myt-1，从而激活MPF，就像海星卵母细胞一样。研究将集中在具有高空间和时间分辨率的光学成像和光谱技术上。 该项目汇集了两个具有互补专业知识和研究能力的实验室的努力。 Kishimoto教授在日本的实验室是世界上研究包括成熟卵子在内的各种细胞中MPF调节的领先中心之一。 该实验室专门研究海星卵的成熟。 该实验室采用低压形式的显微注射，特别适合Akt抗体灭活所需的定量注射。 斯特里克教授的实验室利用了另一种高压显微注射系统，这种系统不容易进行定量。这项研究将为两国共同努力提供一个很好的机会。 通过交流思想和技术，该项目将扩大我们的基础知识，促进国际理解与合作。 该项目通过一名研究生的参与促进国际人力资源