Developing and Disseminating New Laboratories on Plant Molecular Genetics and Genomics

植物分子遗传学和基因组研究仍然落后于以医学为导向的微生物和高等动物研究。因此，在较低的大学水平上，相对较少的实验室经验可以让学生接触到由基因组生物学提供的对植物的日益深入的见解。这个CCLI项目正在开发一个基于实验室和互联网的课程，将学生带到现代植物研究的前沿。该项目主要基于NSF资助的冷泉港实验室(CSHL)主要植物研究的数据，并包括活跃研究人员的大量投入。它基于Dolan DNA学习中心16年来开发和传播创新实验室课程的经验，以及作为互联网网站出版商的经验，这些网站具有强大的多媒体内容和共享使用学生生成的数据。一套全面的基于快速和可重复的聚合酶链式反应化学的实验室是在先前的高级技术教育计划的资助下开发的。利用模式植物拟南芥和重要的粮食作物，实验室阐述了基因和基因组分析的关键概念，包括：表型和分子基因型之间的关系，植物的遗传修饰和食品中转基因的检测，以及用于基因定位的连锁和生物信息学方法。学生也有独特的机会来探索功能基因组学，通过协助CSHL的研究人员对未知功能的拟南芥基因进行细胞分析。一个互联网“超级站点”通过在线协议、定制分析工具、共享数据库、协作公告板和聊天系统来支持实验室。该计划由最初的开发阶段组成，最终是一个焦点研讨会，来自代表美国六个地区的两年制和四年制大学的教师顾问。在传播阶段，教师顾问将组织为期一周的培训研讨会，有144名教师参加。应用生物系统公司、Bio-Link国家生物技术高级技术培训中心、卡罗莱纳生物供应公司和冷泉港实验室出版社致力于为该项目提供关键援助。

Plant molecular genetic and genomic research still lag behind medically-oriented research on microbes and higher animals. As a result, relatively few lab experiences that expose students to the growing insights into plants offered by genomic biology are available at the lower college level. This CCLIproject is developing a laboratory- and Internet-based curriculum to bring students to the forefront of modern plant research. The project is based largely on data emanating from major NSF-funded plant research at Cold Spring Harbor Laboratory (CSHL) and includes substantial input from active researchers. It builds on the Dolan DNA Learning Center's 16 years of experience developing and disseminating innovative laboratory curricula, and its experience as a publisher of Internet sites with strong multimedia content and shared use of student-generated data. A comprehensive set of laboratoriesis based on rapid and reproducible PCR chemistry developedunder a previous grant from the Advanced Technological Education Program. Using the model plant Arabidopsis and important food crops, the laboratories illustrate key concepts of gene and genome analysis, including: the relationship between phenotype and molecular genotype, genetic modification of plants and detection of transgenes in foods, and linkage and bioinformatic methods for gene mapping. Students also have the unique opportunity to explore functional genomics by assisting CSHL researchers with the cellular analysis of Arabidopsis genes of unknown function. An Internet "super site" supports the laboratories with online protocols, custom analysis tools, shared databases, and collaborative bulletin boards and chat systems.The proposal is comprised of an initial development phase, culminating in a focus workshop offaculty advisors drawn from two- and four-year colleges representing six regions of the United States.During the dissemination phase, faculty advisors will organize weeklong training workshops to reach 144 instructors. Applied Biosystems, Bio-Link National Center for Advanced Technological Training in Biotechnology, Carolina Biological Supply Company, and Cold Spring Harbor Laboratory Press are committed to provide key assistance to the project.