A Partnership to Develop Advanced Technology Units on Genomic Biology

我们建议开发和传播基因组生物学的先进技术单元，这将进一步培养高中生和大专学生参与应用生物学的未来。这些单元建立在强大的科学内容和实践技能的基础上，同时鼓励学生继续进行正式的科学培训，并为高级技术职业做好更好的准备。这些单元强调遗传信息中的进化相似性和个体差异的基本主题。现代基因鉴定和分析方法以学生对人类、昆虫和植物的DNA多态(“指纹”)的实验为例进行说明。比较基因组学和进化生物学通过基因数据库的计算机操作来说明--包括使用学生生成的数据的在线项目，以及来自DNA/蛋白质数据库的数据。学生还有机会准备一个关键的酶(Taq聚合酶)和构建一个关键的实验室仪器(DNA热循环仪)。人类基因多态和数据库操作的实验室实验也自然地导致了对基因测试/数据库建立的伦理、法律和社会问题的讨论。该项目是冷泉港实验室DNA学习中心(DNALC)、基因组研究所科学教育基金会(TIGR SEF)、职业研究与发展中心(CORD)以及高中和大专教职员工之间的合作伙伴关系。课程材料将基于DNALC和芝加哥大学开发的关键实验室和计算机技术，使学生可以使用和负担得起聚合酶链式反应(PCR)的DNA指纹。高中单元侧重于相对简单的人类多态和统计分析，明确地与CORD在生物/化学(ABC)课程中的全国应用相结合。在此背景下，该项目可以提升ABC课程的智力内容，并促进高等教育的协调后续教学。学院单元扩展到更雄心勃勃的线粒体DNA序列多态分析，保护和进化生物学的开放式实验，以及生物之间DNA/蛋白质序列比较的高级统计。高级练习将基于TIGR的信息学软件；广泛收集人类表达序列标签(EST)和细菌流感嗜血杆菌、生殖支原体和雅纳什甲烷球菌的完整DNA序列。其中包括由合作机构提供的一项新的测序服务，用于为学生课堂生成人类线粒体序列。

We propose to develop and disseminate Advanced Technology Units on Genomic Biology that will further prepare high school and junior college students to participate in the future of applied biology. The units are based on strong science content and practical skills that at once encourage students to continue with formal science training and better prepare them for advanced technical vocations. The units stress fundamental themes of evolutionary similarity and individual variation in genetic information. Modern methods of gene identification and analysis are illustrated by student experiments with human, insect, and plant DNA polymorphisms (''fingerprints''). Comparative genomics and evolutionary biology are illustrated by computer manipulations of gene databases -- including on-line projects using student-generated data, as well as data from DNA/protein databases. Students also have the opportunity to prepare a key enzyme (Taq polymerase) and construct a key laboratory instrument (DNA thermal cycler). Laboratory experiments with human polymorphisms and database manipulations also lead naturally to discussion of the ethical, legal, and social issues of genetic testing/databasing. The project is a partnership between the DNA Learning Center (DNALC) of Cold Spring Harbor Laboratory, The Institute for Genomic Research Science Education Foundation ( TIGR SEF), the Center for Occupational Research and Development (CORD), and high school and junior college faculty. The curriculum materials will be based on key lab and computer technology developed at the DNALC and the University of Chicago that makes DNA fingerprinting by polymerase chain reaction (PCR) accessible and affordable for student use. The high school unit, which focuses on relatively simple human polymorphisms and statistical analyses, expressly articulates with CORD's nationally used Applications in Biology/Chemistry (ABC) curriculum. In this context, the project can upgrade the intellectual content of the ABC curriculum and fa cilitate coordinated follow-on instruction in higher education. The college unit extends to more ambitious analysis of sequence polymorphisms in mitochondrial DNA, open-ended experiments in conservation and evolutionary biology, and advanced statistics for DNA/protein sequence comparisons between organisms. Advanced exercises will be based on TIGR's informatics software; an extensive collection of human expressed sequence tags (ESTs) and complete DNA sequences of the bacteria Haemophilus influenzae, Mycoplasma genitalium, and Methanococcus janaschii. Included is a novel Sequencing Service by the partner institutions to generate human mitochondrial sequence for student classes.