Selbstorganisation von Nanopartikeln an der Grenzfläche von gemischten Lipid/Polymer-Membranen und Polymersomen

混合脂质/聚合物膜和聚合物囊泡界面处纳米颗粒的自组装

• 批准号: 161161729
• 负责人: Professor Dr. Wolfgang Binder
• 金额: --
• 依托单位: Arbeitsbereich Technische Chemie und Makromolekulare Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161161729?language=en
• 关键词: Selbstorganisation; von; Nanopartikeln; der; Grenzfl

The project deals with the synthesis of triphilic blockcopolymers and their self-assembly in mixed lipid/polymer bilayer membranes, with a focus on controlling the domain-sizes in such mixed membranes as well as the introduction of biologically significant folding-parts. The conformation of the polymer chains together with the polymers hydrophobicity will be controlled by linkage with an artificial folding element (the so called beta-turn), which – together with a hydrogen bond – will lead to a conformational restriction of the two, incompatible polymerchains. The blockcopolymer will consist of poly(isobutylene) as hydrophobic part, and either a fluorophilic poly(oxazoline) or a helical poly(isocyanide) or poly(isocyanate), linked via a lowmolecular (nonpeptidic) beta-turn element, so as to enable the “folding” of both incompatible polymerchains into a lipid-bilayer membrane. The polymers will be synthesized via living polymerization living (ring-opening) carbocationic polymerization or via the living polymerization of isocyanides/isocyanates, the beta-turn-folding element will be introduced via azide/alkyne-“click”-chemistry. Subsequently, the self-assembly of these blockcopolymers in mixed lipid/polymer bilayer-membranes as well as monolayer membranes to control and influence the domain sizes will be investigated, most of all via confocal-laser-microscopy (CFLM). In order to achieve a switchable behavior of self-assembly behavior, a switchable beta-turn element will be introduced, thus allowing to modulate the domain-sizes in mixed lipid/polymer bilayer membranes via external stimuli.

该项目涉及三亲性嵌段共聚物的合成及其在混合脂质/聚合物双层膜中的自组装，重点是控制这种混合膜中的结构域大小，以及引入具有生物学意义的折叠部件。聚合物链的构象以及聚合物的疏水性将通过与人工折叠元素（所谓的β -转）的连接来控制，该元素与氢键一起将导致两种不相容的聚合物的构象限制。嵌段共聚物将由作为疏水部分的聚（异丁烯）和亲氟聚（恶唑啉）或螺旋聚（异氰酸酯）或聚（异氰酸酯）组成，通过低分子（非肽）β -旋元连接，从而使两种不相容的聚物“折叠”成脂质双层膜。聚合物将通过活性聚合（开环）碳阳离子聚合或异氰酸酯/异氰酸酯的活性聚合合成，通过叠氮化物/炔-“点击”化学引入β -转折叠元素。随后，将研究这些嵌段共聚物在混合脂质/聚合物双层膜和单层膜中的自组装，以控制和影响畴大小，主要是通过共聚焦激光显微镜（CFLM）进行。为了实现自组装行为的可切换行为，将引入可切换的β -turn元件，从而允许通过外部刺激调节混合脂质/聚合物双层膜中的畴大小。