Selbstorganisation von Nanopartikeln an der Grenzfläche von gemischten Lipid/Polymer-Membranen und Polymersomen

混合脂质/聚合物膜和聚合物囊泡界面处纳米颗粒的自组装

• 批准号: 161161729
• 负责人: Professor Dr. Wolfgang Binder
• 金额: --
• 依托单位: Arbeitsbereich Technische Chemie und Makromolekulare Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161161729?language=en
• 关键词: Selbstorganisation; von; Nanopartikeln; der; Grenzfl

The project deals with the synthesis of triphilic blockcopolymers and their self-assembly in mixed lipid/polymer bilayer membranes, with a focus on controlling the domain-sizes in such mixed membranes as well as the introduction of biologically significant folding-parts. The conformation of the polymer chains together with the polymers hydrophobicity will be controlled by linkage with an artificial folding element (the so called beta-turn), which – together with a hydrogen bond – will lead to a conformational restriction of the two, incompatible polymerchains. The blockcopolymer will consist of poly(isobutylene) as hydrophobic part, and either a fluorophilic poly(oxazoline) or a helical poly(isocyanide) or poly(isocyanate), linked via a lowmolecular (nonpeptidic) beta-turn element, so as to enable the “folding” of both incompatible polymerchains into a lipid-bilayer membrane. The polymers will be synthesized via living polymerization living (ring-opening) carbocationic polymerization or via the living polymerization of isocyanides/isocyanates, the beta-turn-folding element will be introduced via azide/alkyne-“click”-chemistry. Subsequently, the self-assembly of these blockcopolymers in mixed lipid/polymer bilayer-membranes as well as monolayer membranes to control and influence the domain sizes will be investigated, most of all via confocal-laser-microscopy (CFLM). In order to achieve a switchable behavior of self-assembly behavior, a switchable beta-turn element will be introduced, thus allowing to modulate the domain-sizes in mixed lipid/polymer bilayer membranes via external stimuli.

该项目涉及三亲嵌段共聚物的合成及其在混合脂质/聚合物双层膜中的自组装，重点是控制这种混合膜中的结构域大小以及引入具有生物意义的折叠部分。聚合物链的构象和疏水性将通过与人工折叠元件(所谓的β-转角)的连接来控制，这与氢键一起将导致两个不相容的聚合链的构象限制。嵌段共聚物将由聚异丁烯作为疏水部分，以及通过低分子(非肽)β-转角元件连接的亲氟性聚恶唑啉、螺旋聚异氰酸酯或聚异氰酸酯组成，从而使两个不相容的聚合链能够“折叠”成脂质双层膜。聚合物将通过活性聚合活性(开环)碳阳离子聚合或异氰酸酯/异氰酸酯活性聚合合成，β-转折元件将通过叠氮/炔-“点击”化学引入。随后，这些嵌段共聚物在混合脂质/聚合物双层膜以及单层膜中的自组装以控制和影响结构域大小将被研究，主要是通过共聚焦激光显微镜(CFLM)。为了实现可切换的自组装行为，将引入可切换的β转角元件，从而允许通过外部刺激来调节脂质/聚合物混合双层膜中的结构域大小。