U.S. - Swiss Cooperative Research: Linking Adhesion--Promoted Reporter Gene Expression to Bacterial Attachment-Detachment Dynamics in Porous Media

这个为期一年的奖项支持在瑞士日内瓦、伯尔尼和苏黎世的首次休假，以调查细菌在分解污染土壤和水中污染物中的作用。该项目涉及犹他大学的威廉·约翰逊和他的研究生以及三名瑞士研究人员。他们的研究集中在观察细菌附着-分离动力学在良好表征的系统。利用绿色荧光蛋白方法，他们提出表征细菌细胞表面特性，并将这种关系应用于细菌如何通过多孔介质附着和分离。在不同的环境条件下（水流速、细菌表面特征、基质表面粗糙度和溶液化学），将检测选定菌株在不同表面上的附着-分离行为。这个国际合作项目汇集了具有互补专业知识的研究人员。约翰逊教授将与瑞士环境技术研究所的微生物学家保罗·兰迪尼博士一起对细菌细胞表面特性进行表征。伯尔尼大学的Jaro Ricka博士将对细菌附着-分离动力学进行直接观察。将与日内瓦大学的michael Borkovec博士一起开发相互作用和粘附力剖面。了解细菌在多孔介质中的附着和脱离过程在环境和污染清理中具有实际应用价值，特别是可以引入新的方法将污染食菌引入污染场所。

0239482JohnsonThis one-year award supports a first-time sabbatical in Geneva, Bern, and Zurich, Switzerland to investigate the role of bacteria in decomposing pollutants in contaminated soils and waters. The project involves William B. Johnson of the University of Utah and his graduate student and three Swiss researchers. Their research focuses on observations of bacterial attachment-detachment dynamics in well-characterized systems. Using green fluorescence protein methodology, they propose to characterize bacterial cell surface properties and apply this relationship to how bacteria attach and detach themselves through porous media. The attachment-detachment behavior of selected bacterial strains on various surfaces will be examined under different environmental conditions (water velocity, bacterial surface characteristics, substratum surface roughness and solution chemistry). This international cooperative program brings together researchers with complementary expertise. Professor Johnson will perform characterization of bacterial cell surface properties with Dr. Paolo Landini, a microbiologist at the Swiss Institute for Environment Technology. Direct observation of bacterial attachment-detachment dynamics will be examined with Dr. Jaro Ricka of the University of Bern. Interaction and adhesion force profiles will be developed with Dr. Michal Borkovec at the University of Geneva. Understanding of attachment and detachment processes of bacteria in porous media have practical applications in environmental and pollution clean-up, and in particular, could lead to new methods to introduce pollution eating bacteria into contaminated sites.