CAREER: Advancing MS For Peptide/Protein Sequencing

职业：推进肽/蛋白质测序的 MS

• 批准号: 0239800
• 负责人: Michael Van Stipdonk
• 金额: --
• 依托单位: Wichita State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239800&HistoricalAwards=false
• 关键词: CAREER; Advancing; MS; Peptide; Protein

This CAREER award to Professor Michael Van Stipdonk of Wichita State University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. In this project, mass spectrometry will be further developed and refined for identification of peptides and proteins, a crucial step in efforts to understand metabolic pathways, cellular signaling, and the biochemistry of disease. The sequence of amino acids, the order in which they are linked together, ultimately identifies a peptide or protein. Before mass spectrometry can be used for high-throughput and high-accuracy sequencing directly (i.e. without resorting to library or database searches), a clear understanding of the "rules" that govern peptide and protein dissociation in the gas-phase is required. The project includes a comprehensive investigation of factors that guide dissociation reactions, including the importance of cation choice (proton or metal ion), peptide sequence, tautomerization, cyclization and intramolecular proton transfer. The work will lead to an optimized and validated mass spectrometry approach to peptide/protein identification that can be applied to proteome analysis, biochemistry and molecular biology.The project has, and will continue to include undergraduate students as integral participants in research. From their experiences, new experiments for the determination of amino acid sequence in peptides will be condensed and transformed into novel educational exercises. With regard to the K-12 system, a new program will be developed to address the important issue of science teacher preparation and retention. The PI and several representatives of area public and private high schools envision a comprehensive resource to support science teachers, including the training of new teachers through the Wichita State University Alternative Certification program. In particular, lesson plans, teaching strategies, and exercises in critical thinking will be developed and shared among many new teachers; already, more than 90 teachers have expressed interest in the program. Opportunities will also be extended to high school teachers to spend summer months conducting research at Wichita State University, strengthening their mastery of chemistry content knowledge, and better preparing them to translate and transfer the excitement and relevance of science to their students.

该职业奖授予威奇托州立大学的Michael Van Stipdonk教授，由化学学部的分析和表面化学项目提供支持。在这个项目中，质谱法将进一步发展和完善，用于鉴定多肽和蛋白质，这是了解代谢途径、细胞信号传导和疾病生物化学的关键一步。氨基酸的序列，即它们连接在一起的顺序，最终确定了肽或蛋白质。在质谱法可以直接用于高通量和高精度测序之前（即无需诉诸文库或数据库搜索），需要清楚地了解控制肽和蛋白质在气相解离的“规则”。该项目包括对引导解离反应的因素的全面调查，包括阳离子选择（质子或金属离子），肽序列，互变异构，环化和分子内质子转移的重要性。这项工作将导致优化和验证肽/蛋白质鉴定的质谱方法，可应用于蛋白质组学分析，生物化学和分子生物学。该项目已经并将继续包括本科生作为研究的不可或缺的参与者。从他们的经验，新的实验，以确定氨基酸序列的多肽将浓缩和转化为新的教育练习。关于K-12教育体系，将制定一项新计划，以解决培养和留住科学教师的重要问题。PI和当地公立和私立高中的几位代表设想了一个全面的资源来支持科学教师，包括通过威奇托州立大学替代认证计划培训新教师。特别是，课程计划、教学策略和批判性思维练习将在许多新教师之间开发和分享；目前已有90多名教师表示对该项目感兴趣。高中教师也将有机会利用暑期时间在威奇托州立大学进行研究，加强他们对化学内容知识的掌握，并更好地准备将科学的兴奋和相关性转化给学生。