Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
植物防御反应中水杨酸结合蛋白的表征
基本信息
- 批准号:0241531
- 负责人:
- 金额:$ 18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-08-15 至 2005-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Plant disease causes an estimated loss worldwide of $100B annually. A variety of strategies are being developed to protect plants against disease. One approach is to induce the plant's own natural defenses. This strategy is attractive since it can provide protection against a broad spectrum of pathogens. Broad-spectrum resistance to a wide array of pathogens, know as systemic acquired resistance (SAR), can be activated by infection with a pathogen or treatment with SAR-inducing natural chemicals such as salicylic acid (SA) and its synthetic functional analogues, INA or BTH. The development of strategies that control disease by manipulating endogenous plant defense responses will be very important for sustaining agricultural production and improving our environment and health.It is now well established that SA plays a key role in the development of resistance in many, but not all, plant-pathogen systems. While our understanding of SA function in disease resistance is far from complete, it is becoming increasingly clear that its role is complex. This increase in the complexity of SA functions in defense is matched by the discovery in tobacco of multiple proteins with which SA interacts. An understanding of SA functions in defense against microbial pathogens will require the identification and characterization of these proteins, the major objective of this research.The overall objective of this proposal is to characterize two SA-binding proteins, SABP2 and SABP3 from tobacco. SABP2 is a very low abundance lipase whose activity is stimulated by SA binding. Silencing of SABP2 suppresses several disease resistance responses. Major emphasis is placed on defining SABP2's role in defense responses. A second objective is to begin an analysis to determine which member(s) of the 18-member Arabidopsis family of SABP2-like proteins corresponds to the tobacco SABP2. Preliminary characterization of SABP2's enzymatic activity, a third objective, will be continued. The fourth objective involves further characterizing SABP3. This protein is the chloroplastic carbonic anhydrase (CA), and based on yeast complementation analysis, it appears to have antioxidant activity. Initial studies have indicated that silencing SABP3 expression alters the HR and local resistance to several pathogens. These findings will be extended by assessing the effects of SABP3/CA silencing on defense gene expression and resistance to other pathogens in Arabidopsis, as well as in tobacco.
据估计,全世界每年因植物疾病造成的损失高达1000亿美元。人们正在开发各种策略来保护植物免受疾病的侵袭。一种方法是诱导植物自身的自然防御。这一策略很有吸引力,因为它可以提供对广泛病原体的保护。对一系列病原体的广谱抗性,称为系统获得性抗性(SAR),可通过感染病原体或使用导致SAR的天然化学物质,如水杨酸(SA)及其合成功能类似物INA或BTH来激活。开发通过操纵内源植物防御反应来控制疾病的策略对于维持农业生产、改善我们的环境和健康将是非常重要的。现在已经证实,SA在许多(但不是所有)植物-病原菌系统中的抗性发展中起关键作用。虽然我们对SA在抗病中的功能的了解还很不完整,但它的作用是复杂的,这一点越来越明显。在烟草中发现了与SA相互作用的多种蛋白质,这与SA防御功能的复杂性的增加相匹配。为了了解SA在防御微生物病原体中的功能,需要鉴定和鉴定这些蛋白质,这是本研究的主要目标。本研究的总体目标是从烟草中鉴定两种SA结合蛋白,SABP2和SABP3。SABP2是一种极低丰度的脂肪酶,其活性受SA结合的刺激。沉默SABP2可以抑制几种抗病反应。主要重点是确定SABP 2在防御反应中的S角色。第二个目标是开始分析,以确定拟南芥类SABP2蛋白家族18个成员中的哪个成员(S)与烟草SABP2相对应。第三个目标是对SABP 2的S酶活性的初步表征,这将继续进行。第四个目标涉及进一步确定SABP3的特征。这种蛋白质是氯塑料碳酸酐酶(CA),根据酵母互补分析,它似乎具有抗氧化活性。初步研究表明,沉默SABP3的表达改变了HR和局部对几种病原体的抵抗力。这些发现将通过评估SABP3/CA沉默对拟南芥和烟草中防御基因表达和对其他病原菌的抗性的影响而得到扩展。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Daniel Klessig其他文献
Daniel Klessig的其他文献
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{{ truncateString('Daniel Klessig', 18)}}的其他基金
Arabidopsis 2010: The Arabidopsis salicylic acid signaling network: A paradigm for phytohormone signaling
拟南芥 2010:拟南芥水杨酸信号网络:植物激素信号传导的范例
- 批准号:
0820405 - 财政年份:2009
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Genetic, molecular and biochemical basis of resistance to turnip crinkle virus in Arabidopsis
拟南芥抗萝卜皱病毒的遗传、分子和生化基础
- 批准号:
0641576 - 财政年份:2007
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of the High Affinity Salicylic Acid-Binding Protein 2 in Plant Disease Resistance
高亲和力水杨酸结合蛋白 2 在植物抗病性中的表征
- 批准号:
0525360 - 财政年份:2005
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of Signal Transduction Pathways in Plant Defense Responses
植物防御反应中信号转导途径的表征
- 批准号:
0110404 - 财政年份:2001
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
植物防御反应中水杨酸结合蛋白的表征
- 批准号:
0110272 - 财政年份:2001
- 资助金额:
$ 18万 - 项目类别:
Standard Grant
Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
植物防御反应中水杨酸结合蛋白的表征
- 批准号:
0196046 - 财政年份:2000
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
植物防御反应中水杨酸结合蛋白的表征
- 批准号:
9904660 - 财政年份:1999
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of the Salicylic Acid Signal Transduction Pathway in Plant Defense Responses
植物防御反应中水杨酸信号转导途径的表征
- 批准号:
9723952 - 财政年份:1997
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Involvement of Salicylic Acid Inhibition of Catalase and Ascorbate Peroxidase in Plant Defense Responses
水杨酸抑制过氧化氢酶和抗坏血酸过氧化物酶参与植物防御反应
- 批准号:
9514239 - 财政年份:1996
- 资助金额:
$ 18万 - 项目类别:
Standard Grant
Characterization of the Salicylic Acid Signal Transduction Pathway in Plant Defense Responses
植物防御反应中水杨酸信号转导途径的表征
- 批准号:
9310371 - 财政年份:1993
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
相似海外基金
Purification and Characterization of Copper Chaperone involved in Salicylic Acid Perception.
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Purification and Characterization of Copper Chaperone involved in Salicylic Acid Perception.
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Characterization of perception mechanism of plant immune hormone using an salicylic acid agonist isolated from a chemical screening
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Characterization of the High Affinity Salicylic Acid-Binding Protein 2 in Plant Disease Resistance
高亲和力水杨酸结合蛋白 2 在植物抗病性中的表征
- 批准号:
0525360 - 财政年份:2005
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Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
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Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
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0196046 - 财政年份:2000
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of the Salicylic Acid Signal Transduction Pathway in Plant Defense Responses
植物防御反应中水杨酸信号转导途径的表征
- 批准号:
0196168 - 财政年份:2000
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of Salicylic Acid-Binding Proteins in Plant Defense Responses
植物防御反应中水杨酸结合蛋白的表征
- 批准号:
9904660 - 财政年份:1999
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of the Salicylic Acid Signal Transduction Pathway in Plant Defense Responses
植物防御反应中水杨酸信号转导途径的表征
- 批准号:
9723952 - 财政年份:1997
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant
Characterization of the Salicylic Acid Signal Transduction Pathway in Plant Defense Responses
植物防御反应中水杨酸信号转导途径的表征
- 批准号:
9310371 - 财政年份:1993
- 资助金额:
$ 18万 - 项目类别:
Continuing Grant