Genetic, molecular and biochemical basis of resistance to turnip crinkle virus in Arabidopsis

PI-Daniel F.Klessigproposal No.IOS-0641576提案的标题：拟南芥对芜菁皱缩病毒的抗性的遗传、分子和生化基础摘要植物病害每年对农业生产和粮食供应造成毁灭性的影响。植物病毒是许多农作物种植的主要威胁，在世界范围内造成严重的经济损失。多年来，这些病原菌一直通过常规的植物保护措施进行控制，如轮作、销毁病源、培育抗性品种、对相关病媒进行化学防治。本项目将研究拟南芥与芜菁皱纹病毒(TCV)之间的相互作用，以阐明植物对病毒侵染的抗性机制。这项研究的总体目标将是剖析对TCV的过敏反应和抗性所需的信号的分子和生化基础。这将包括通过克隆和鉴定假定的TCV抗性抑制因子来确定TCV抗性的遗传基础。此外，还将对几个TCV外壳蛋白识别缺陷突变体进行进一步的鉴定，并研究SA介导的抗性基因表达调控的分子和生化基础。这项工作将为促进宿主和病原体之间的通信的复杂防御信号网络提供新的和重要的见解。通过阐明植物感知和抵抗病毒感染的机制(S)，可能会开发出成功的工程和/或操纵抗病的策略。这项研究的其他更广泛的影响包括培养高中、本科生、研究生和博士后。这项工作的一个重要目标将是培养一个本科生社区，特别是那些来自传统上代表不足的群体的学生，通过将他们纳入科学团队的成员。此外，将努力将研究与肯塔基大学提供的推广服务结合起来，以提高农民和农业企业代表对作物抗药性最新进展的认识。

PI- Daniel F. KlessigProposal No. IOS- 0641576Title of the proposal: Genetic, molecular and biochemical basis of resistance to Turnip Crinkle Virus in ArabidopsisAbstractPlant diseases have a devastating impact on agricultural production and food supply every year. Plant viruses are a major threat to the cultivation of many agricultural crops, causing serious economic losses worldwide. For many years, these pathogens have been controlled by conventional plant protection methods such as crop rotation, destruction of infected sources breeding for resistance, and chemical control of relevant insect vectors. This project will involve study of interaction between Arabidopsis thaliana and Turnip Crinkle Virus (TCV), with an aim to elucidate mechanisms governing resistance of plants to viral infections. The overall goal of this research will be to dissect the molecular and biochemical basis of signaling required for hypersensitive response and resistance to TCV. This will include determining the genetic basis of resistance to TCV by cloning and characterization of a putative suppressor of resistance to TCV. In addition, several TCV coat protein recognition defective mutants will be characterized further and molecular and biochemical basis of SA-mediated regulation of resistance gene expression will be studied. This work will provide novel and important insights into the complex defense signaling networks that facilitate communication between host and pathogen. By elucidating the mechanism(s) through which plants perceive and resist viral infection, successful strategies for engineering and/or manipulating disease resistance may be developed. Other broader impacts of this study include training of high school, undergraduate, graduate and postdoctoral students. One important aim of this work will be to foster a community of undergraduate students, particularly those from traditionally underrepresented groups, by including them as members of the scientific team. In addition, attempts will be made to integrate research with the extension services available at the University of Kentucky to enhance awareness of the recent advances in crop resistance among farmers and agribusiness representatives.