Arabidopsis 2010: The Arabidopsis salicylic acid signaling network: A paradigm for phytohormone signaling

拟南芥 2010：拟南芥水杨酸信号网络：植物激素信号传导的范例

• 批准号: 0820405
• 负责人: Daniel Klessig
• 金额: $ 237.88万
• 依托单位: Boyce Thompson Institute Plant Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0820405&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; salicylic; acid; signaling

The plant hormone salicylic acid (SA) affects many diverse processes in plant growth and development and plays important role(s) in response to abiotic and biotic stresses. Its role(s) in disease resistance has been the most extensively studied but is still only partially understood. To obtain insights into how SA carries out its varied functions, a large number of putative SA-binding proteins (pSABPs) have recently been identified in Arabidopsis by affinity chromatography using SA attached to a matrix. The investigators will use two powerful high throughput screens, an Arabidopsis protein micro-array and a yeast three hybrid system, to identify additional SABPs, as well as confirm the SA-binding activity of the already identified pSABPs. These SABPs will be characterized using biochemical, genetic, molecular, bioinformatic, and biophysical (X-ray crystallography) techniques to decipher their functions in growth and development and/or responses to abiotic and biotic stresses, and the effects of SA binding on these functions. Based on current data and the results obtained, an SA signaling network model(s) will be constructed, tested by more detailed characterization of putative signaling or regulatory modules, and refined in order to produce an exemplary model of the SA signaling network of SABPs which may serve as a paradigm for other plant hormones (and other small ligands). The project will also facilitate development of the two new, less proven approaches for large scale identification of proteins that bind small molecules (like hormones), for their use in characterization of other hormones or small molecule signaling networks in animals as well as plants. Moreover, the SA targets (SABPs) identified in plants may have counterparts in humans that are important in mediating SA's and aspirin's (aceto SA) diverse therapeutic properties including reduction in fever, pain, inflammation, and risk of heart attack, stroke and cancer. The project will provide a stimulating training environment for graduate and postdoctoral students, where they are exposed to a large repertoire of biochemical, molecular, genetic, structural, and bioinformatic approaches to address fundamental questions in plant biology. It also will provide high school students and undergraduates with opportunities to experience first-hand the excitement of discovery under the close mentorship of a postdoctoral fellow, research associate or graduate student. Research results will be disseminated widely through peer-reviewed articles in journals that allow broad electronic access. Moreover, the identity of new SABPs (once their binding is verified by two independent assays) and their possible involvement in various plant processes will be made available through a new website before publication in peer-reviewed journals.

植物激素水杨酸（SA）影响植物生长发育的许多不同过程，并在响应非生物和生物胁迫中发挥重要作用。它在抗病性中的作用已被最广泛地研究，但仍然只有部分了解。为了深入了解SA如何执行其不同的功能，大量的推定SA结合蛋白（pSABPs）最近已被确定在拟南芥亲和层析使用SA连接到一个矩阵。研究人员将使用两个强大的高通量筛选，一个拟南芥蛋白微阵列和一个酵母三杂交系统，来鉴定其他SABP，并确认已经鉴定的pSABP的SA结合活性。这些SABP将使用生物化学，遗传，分子，生物信息学和生物物理（X射线晶体学）技术来表征，以破译它们在生长和发育和/或对非生物和生物胁迫的响应中的功能，以及SA结合对这些功能的影响。基于当前的数据和获得的结果，将构建SA信号传导网络模型，通过对推定的信号传导或调节模块进行更详细的表征来测试，并进行改进以产生SABP的SA信号传导网络的示例性模型，其可以用作其他植物激素（和其他小配体）的范例。该项目还将促进开发两种新的、未经证实的方法，用于大规模鉴定结合小分子（如激素）的蛋白质，用于表征动物和植物中的其他激素或小分子信号网络。此外，在植物中鉴定的SA靶标（SABP）可能在人类中具有对应物，其在介导SA和阿司匹林（aceto SA）的多种治疗特性中是重要的，包括减少发烧、疼痛、炎症以及心脏病发作、中风和癌症的风险。该项目将为研究生和博士后学生提供一个刺激的培训环境，让他们接触到大量的生物化学，分子，遗传，结构和生物信息学方法，以解决植物生物学中的基本问题。它还将为高中生和本科生提供机会，在博士后、研究助理或研究生的密切指导下，亲身体验发现的兴奋。研究结果将通过同行审查的文章在允许广泛电子访问的期刊上广泛传播。此外，新的SABP的身份（一旦其结合被两个独立的测定验证）及其可能参与各种植物过程将通过一个新的网站提供，然后在同行评审的期刊上发表。