Collaborative Research: Role of Meso- and Bathypelagic prokaryotic Organisms in the Marine Water Column: Insights from Compound-Specific Radiocarbon Analysis

合作研究：海洋水体中中层和深海原核生物的作用：化合物特异性放射性碳分析的见解

• 批准号: 0242160
• 负责人: Lihini Aluwihare
• 金额: $ 16.08万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0242160&HistoricalAwards=false
• 关键词: Collaborative; Research; Role; Meso; Bathypelagic

ABSTRACTOCE-0242160OCE-0241363Even though the meso- and bathypelagic regions beneath the euphotic zone contain nearly all of the oceans volume, little is known about the biogeochemical processes that function in this region. For this reason, a PI from Scripps Institute of Oceanography will collaborate with a PI from Harvard University to determine which carbon sources fuel prokaryotic production in the meso- and bathypelagic ocean. Specifically, the PIs wish to address the following two questions: (1) Is freshly-produced organic matter the primary dissolved organic carbon (DOC) pool that is accessible to deep ocean heterotrophic prokaryotes or is "old" DOC also bioavailable?; and (2) Is chemoautotrophic fixation of inorganic carbon by planktonic archaea an important component of the deep ocean carbon cycle? To address these questions, the PIs plan to measure the radiocarbon (14C) concentration of the carbon source pools and the resulting prolaryotic (bacterial and archaeal) biomass at the surface, meso (500-600 m) and bathypelagic (900 m) depths. In addition, fluorescent in-situ hybridization (FISH) and genetic diversity analysis by denaturing gradient gel electrophorsis (DGGE) will provide a complementary picture of the biological community. Lastly, to obtain the large quantities of biomass-derived carbon needed for this study, the PIs plan to develop an in-situ, battery powered, submersible pump capable of pumping 20,000L of seawater per deployment during the first year of the study.

尽管真光层之下的中层和深层区域几乎包含了所有的海洋体积，但人们对这一区域的地球化学过程知之甚少。 出于这个原因，斯克里普斯海洋研究所的一名PI将与哈佛大学的一名PI合作，以确定哪些碳源为中层和深海的原核生物生产提供燃料。 具体而言，研究者希望解决以下两个问题：（1）新鲜产生的有机物是深海异养原核生物可利用的主要溶解有机碳（DOC）库，还是“老”DOC也可生物利用？（2）浮游古菌对无机碳的化能自养固定是深海碳循环的重要组成部分吗？ 为了解决这些问题，项目研究员计划测量碳源池的放射性碳（14 C）浓度以及由此产生的表层、中层（500-600米）和深海（900米）深度的原核生物（细菌和古细菌）生物量。 此外，荧光原位杂交（FISH）和变性梯度凝胶电泳（DGGE）的遗传多样性分析将提供一个生物群落的补充图片。 最后，为了获得本研究所需的大量生物质衍生碳，PI计划在研究的第一年内开发一种现场电池供电的潜水泵，每次部署能够泵送20，000 L海水。