MRI: Acquisition of Instuments to Facilitate Molecular-Level Studies in Earth and Ocean Sciences

该提案要求提供资金，用于配备预备级分收集器的气相色谱飞行时间质谱仪和配备多种样品电离模式和分析级分收集器的液相色谱线性离子阱质谱仪。 PI和她的学生将使用该仪器来表征和纯化复杂环境样品中的生物分子，用于化学，生物和地质研究工作。 该设施将用于支持SIO现有和拟议的研究工作，包括鉴定与深海细菌的温度和压力耐受性相关的膜脂和蛋白质修饰，以及基因组学支持的膜脂生物合成研究。 该设施还将用于开发新的分析方法，并对研究生进行仪器操作和使用方面的培训。 更广泛的影响PI在通过接待女高中生来促进妇女参与科学方面有着良好的记录。 拟议的仪器将补充和扩大SIO研究人员以及邀请的外部研究人员的能力。 将举办海洋化学和地球化学或海洋生物学系列研讨会，向更广泛的SIO人群展示设施能力和结果

The proposal requests funds for a gas-chromatograph time-of-flight mass spectrometer with a preparatory fraction collector and a liquid chromatograph-linear ion trap mass spectrometer with multiple sample ionization modes and an analytical fraction collector. The instrumentation will be used by the PI and her students to characterize and purify biomolecules from complex environmental samples for chemical, biological and geological research efforts. The facility will be used to support existing and proposed research efforts at SIO, including identification of membrane lipid and protein modifications associated with temperature and pressure tolerance of deep-ocean bacteria, and genomics enabled studies of membrane lipid biosynthesis. The facility will also be used to develop new analytical methods, and train graduate students in instrumentation operation and use. Use of the facility by outside users is by invitation.Broader impactsThe PI has a strong record of enhancing the participation of women in science by hosting female high school students. The proposed instrumentation will complement and expand the capabilities of SIO researchers, as well as invited outside researchers. Seminars will be held for either the Marine Chemistry and Geochemistry or Marine Biology seminar series demonstrating the facility capabilities and results to the larger SIO populace