MRI: Acquisition of instrumentation to advance our understanding of environmental processes through characterization of the organic compounds in different environmental reservoirs

MRI：购买仪器，通过表征不同环境库中的有机化合物来加深我们对环境过程的理解

• 批准号: 1531350
• 负责人: Lihini Aluwihare
• 金额: $ 33.31万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531350&HistoricalAwards=false
• 关键词: MRI; Acquisition; instrumentation; advance; our

This project will purchase an off-the-shelf instrument that has an unprecedented capability to separate individual molecules found in complex matrices and more precisely access their composition (through accurate measurement of mass). The environmental matrices that will be studied in this research and the innovative sample processing methods that will be employed to reduce the natural complexity of relevant samples represents a novel application of this instrument. Examples of new knowledge that will be created include a better understanding of how contaminants are transformed in the environment, how microscopic organisms regulate bloom dynamics through communication, and how known biochemicals are transformed into molecules that can be preserved on long timescales, thus contributing to the production of oxygen and the sequestration of carbon dioxide. Such a molecular-level understanding of these processes is essential for determining the environmental factors that regulate them and how these processes may respond in the future. The data produced with this instrument will be publically available as a mass spectral library that will be of use to other fields including drug discovery, petroleum research, and metabolomics. In addition, several graduate students and post doctoral researchers will receive hands-on access to this instrument, providing valuable training that will prepare them well for careers in both academia and industry.This project will acquire a comprehensive gas chromatograph (GC×GC) coupled to a Pegasus HRT (High Resolution TOF) Mass Spectrometer (MS), which will be initially utilized to examine the diversity of organic molecules accumulating in aquatic environments and also to identify degradation products of chemical contaminants accumulating in various environment matrices. Organic geochemistry and environmental chemistry necessarily deals with complex matrices where thousands to millions of compounds derived from various sources, acted upon by abiotic and biotic processes, accumulate on short to millennial (and beyond) timescales. Chemical structures of these compounds carry critical information regarding source, mechanisms of transformation and residence time. Access to advanced analytical instrumentation continues to increase access to such information. Comprehensive gas chromatography, together with advanced mass deconvolution afforded by the high scan rates of the identified instrument (200 full mass range mass spectra per second) enables unprecedented ?separation? of complex mixtures of organic molecules. Additionally, the sub-ppm mass accuracy greatly enhances the likelihood of unknown compound identification. Together these two features make this instrument unique. Knowledge about the processes that shape the chemical composition of various environmental reservoirs will scale with the extent to which these complex samples can be chemically described. As such, there is a significant reward associated with comprehensively characterizing the chemical composition of such samples.

该项目将购买一台现成的仪器，该仪器具有前所未有的能力，可以分离复杂基质中的单个分子，并更精确地获取其组成（通过精确测量质量）。在这项研究中将研究的环境矩阵和创新的样品处理方法，将用于降低相关样品的自然复杂性，代表了这种仪器的一种新的应用。将创造的新知识的例子包括更好地了解污染物如何在环境中转化，微生物如何通过通信调节水华动态，以及已知的生物化学物质如何转化为可以在长时间尺度上保存的分子，从而有助于氧气的产生和二氧化碳的封存。对这些过程的这种分子水平的理解对于确定调节它们的环境因素以及这些过程在未来如何响应是至关重要的。用这种仪器产生的数据将作为质谱库，可用于其他领域，包括药物发现，石油研究和代谢组学。此外，一些研究生和博士后研究人员将获得该仪器的实际操作机会，提供有价值的培训，使他们为学术界和工业界的职业生涯做好准备。该项目将获得一台与Pegasus HRT耦合的综合气相色谱仪（GC×GC）（高分辨率TOF）质谱仪（MS），这将首先用于研究在水生环境中积累的有机分子的多样性，并确定在各种环境中积累的化学污染物的降解产物。环境矩阵 有机地球化学和环境化学必然涉及复杂的基质，其中来自各种来源的数千至数百万种化合物，受到非生物和生物过程的作用，在短至千年（甚至更长）的时间尺度上积累。这些化合物的化学结构携带关于来源、转化机制和停留时间的关键信息。先进的分析仪器的使用继续增加了获得此类信息的机会。全面的气相色谱分析，加上先进的质量去卷积所提供的高扫描速率的识别仪器（200全质谱范围每秒），使前所未有的？分居？有机分子的复杂混合物。此外，亚ppm质量精度大大提高了未知化合物识别的可能性。这两个特点使该仪器独一无二。关于塑造各种环境储层的化学成分的过程的知识将随着这些复杂样品可以被化学描述的程度而扩展。因此，全面表征这些样品的化学组成是一个重要的回报。