The role of PAM2 proteins during polar growth of U. maydis

PAM2蛋白在玉米极地生长过程中的作用

• 批准号: 161738550
• 负责人: Professor Dr. Michael Feldbrügge
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161738550?language=en
• 关键词: role; PAM2; proteins; during; polar

Polar growth of filaments of Ustilago maydis depends on long-distance transport of mRNAs. The key RNA-binding protein Rrm4 transports distinct sets of mRNAs as part of ribonucleoprotein (mRNP) complexes. Corresponding mRNAs encode polarity factors e.g. the small G protein Rho3 and the septin Cdc3 as well as mitochondrial proteins. Polar accumulation of Cdc3 at hyphal tips is disturbed in rrm4Δ strains, indicating that transport of target mRNAs determines the correct subcellular localisation of encoded proteins. Although the RNA-binding protein and its cargo mRNAs are known, the exact mechanism(s) of transport remains elusive. Here, we aim to investigate the protein composition of Rrm4- containing mRNPs to further elucidate the mechanism(s) of mRNA trafficking. Since Rrm4 is an integral part of transport mRNPs, it will be used as a molecular handle to purify associated components. Interesting candidates will be further characterised to uncover their function e.g. during RNA binding as well as during formation and movement of mRNP complexes. These studies will allow the identification and functional classification of novel trafficking components and provide deeper insights into the biological processes underlying polar growth in eukaryotes.

玉米黑粉菌菌丝的极性生长依赖于mRNA的长距离运输。关键的RNA结合蛋白Rrm4作为核糖核蛋白（mRNP）复合物的一部分转运不同的mRNA组。相应的mRNA编码极性因子，例如小G蛋白Rho3和隔蛋白Cdc3以及线粒体蛋白。在rrm4Δ菌株中，Cdc3在菌丝顶端的极性积累受到干扰，表明靶mRNA的运输决定了编码蛋白的正确亚细胞定位。虽然RNA结合蛋白及其货物mRNA是已知的，但确切的转运机制仍然难以捉摸。在这里，我们的目的是研究的蛋白质组成的Rrm4含有mRNP进一步阐明的mRNA运输的机制。由于Rrm4是转运mRNP的组成部分，因此它将用作分子手柄以纯化相关组分。将进一步表征感兴趣的候选物以揭示它们的功能，例如在RNA结合期间以及在mRNP复合物的形成和移动期间。这些研究将允许新的贩运组件的识别和功能分类，并提供更深入的了解在真核生物极性生长的生物过程。

项目成果

Establishing a versatile Golden Gate cloning system for genetic engineering in fungi.

建立用于真菌基因工程的多功能金门克隆系统

• DOI: 10.1016/j.fgb.2013.10.012
• 发表时间: 2014
• 期刊: Fungal genetics and biology : FG & B
• 作者: M. Terfrüchte;B. Jöhnk;R. Fajardo-Somera;G. Braus;M. Riquelme;K. Schipper;M. Feldbrügge
• 通讯作者: M. Feldbrügge

Live cell imaging of endosomal trafficking in fungi.

真菌内体运输的活细胞成像

• DOI: 10.1007/978-1-4939-2309-0_24
• 发表时间: 2015
• 期刊: Methods in molecular biology
• 作者: S. Baumann;N. Takeshita;N. Grün;R. Fischer;M. Feldbrügge
• 通讯作者: M. Feldbrügge