Endosomal transport of septin mRNAs and encoded proteins in the phytopathogen Ustilago maydis

植物病原体玉米黑粉菌中 septin mRNA 和编码蛋白的内体转运

• 批准号: 258378373
• 负责人: Professor Dr. Michael Feldbrügge
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/258378373?language=en
• 关键词: Endosomal; transport; septin; mRNAs; encoded

Endosomes transport cellular macromolecules such as lipids and proteins. These molecular carriers mainly receive their cargo from other membrane compartments. Particularly in highly polarised cells like neurons and fungal hyphae, endosomes shuttle along microtubules and carry out sophisticated functions in long-distance transport. Evidence is accumulating that these membranous carriers also transport mRNAs as unconventional cargo. However, little is known about the precise molecular function of this endosomal mRNA transport. In the phytopathogen Ustilago maydis we recently discovered that septin mRNA and protein transport on endosomes is important for correct formation of septin filaments at the hyphal growth pole. We propose a novel cell biological mechanism, namely endosome-coupled translation, which is important for loading of endosomes with septins for their correct assembly and transport. Here, we challenge this hypothesis by investigating local translation in vivo. Moreover, we will address whether all four septins co-localise on endosomes as well as in filaments and test whether this is dependent of mRNA transport. Thus, we will gain new insights in the molecular connection of mRNA transport, membrane trafficking and septin biology.

核内体运输细胞大分子，如脂质和蛋白质。这些分子载体主要从其他膜室接收它们的货物。特别是在高度极化的细胞中，如神经元和真菌菌丝，核内体沿着微管穿梭，在长距离运输中执行复杂的功能。越来越多的证据表明，这些膜载体也作为非常规货物运输mrna。然而，对这种内体mRNA转运的精确分子功能知之甚少。在真菌黑穗病菌（Ustilago maydis）中，我们最近发现核内体上的septin mRNA和蛋白转运对于菌丝生长极septin丝的正确形成至关重要。我们提出了一种新的细胞生物学机制，即核内体偶联翻译，它对核内体的正确组装和运输至关重要。在这里，我们通过研究体内的局部翻译来挑战这一假设。此外，我们将讨论所有四种septin是否在核内体和细丝上共定位，并测试这是否依赖于mRNA运输。因此，我们将在mRNA转运、膜转运和septin生物学的分子连接方面获得新的见解。

项目成果

The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport

• DOI: 10.15252/embr.201847381
• 发表时间: 2019-07-24
• 期刊: EMBO REPORTS
• 影响因子: 7.7
• 作者: Jankowski, Silke;Pohlmann, Thomas;Feldbruegge, Michael
• 通讯作者: Feldbruegge, Michael

Endosomal assembly and transport of heteromeric septin complexes promote septin cytoskeleton formation

异聚septin复合物的内体组装和运输促进septin细胞骨架的形成

• DOI: 10.1242/jcs.182824
• 发表时间: 2016
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: S. Zander;S. Baumann;S. Weidtkamp-Peters;M. Feldbrügge
• 通讯作者: M. Feldbrügge

Polycistronic gene expression in the model micro-organism Ustilago maydis

• DOI: 10.1101/2020.05.06.081414
• 发表时间: 2020-05
• 期刊: bioRxiv
• 作者: K. Müntjes;M. Philipp;Lisa Hüsemann;Nicole Heucken;S. Weidtkamp‐Peters;Kerstin Schipper;Matias D. Zurbriggen;M. Feldbrügge

RNA Live Imaging in the Model Microorganism Ustilago maydis.

模型微生物玉米黑粉菌的 RNA 实时成像

• DOI: 10.1007/978-1-4939-7213-5_21
• 发表时间: 2018
• 期刊: Methods in molecular biology
• 作者: S. Zander;K. Müntjes;M. Feldbrügge
• 通讯作者: M. Feldbrügge

The STRIPAK signaling complex regulates phosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes

• DOI: 10.1101/2020.05.01.072009
• 发表时间: 2020-05
• 期刊: bioRxiv
• 作者: V. Stein;B. Blank-Landeshammer;K. Müntjes;R. Märker;I. Teichert;M. Feldbrügge;A. Sickmann;U. Kück