Crosstalk between PKA and MAPK signalling during mating in Ustilago maydis

玉米黑粉菌交配过程中 PKA 和 MAPK 信号之间的串扰

• 批准号: 5431170
• 负责人: Professor Dr. Michael Feldbrügge
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5431170?language=en
• 关键词: Crosstalk; between; PKA; MAPK; signalling

Crosstalk between protein kinase A (PKA) and mitogen-activated protein kinase (MAPK) in the corn pathogen Ustilago maydis serves as a model for eukaryotic signalling networks. In this basidiomycete PKA/MAPK crosstalk is crucial for pheromone signalling during mating, which is prerequisite for pathogenic development. Recently, we described a novel mechanism of crosstalk by demonstrating that the activity of the key transcription factor Prf1 is regulated by PKA as well as MAPK phosphorylation. The differential phosphorylation by these kinases enables Prf1 to discriminate various target gene promoters. In the proposed project we wish to answer the question how the activity of Prf1 is regulated by PKA as well as MAPK phosphorylation. In addition, since MAPK signalling is needed for a specific subset of target genes we want to address the hypothesis that a second transciption factor acts in concert with MAPK-phosphorylated Prf1.

玉米黑粉菌中蛋白激酶A（PKA）和丝裂原活化蛋白激酶（MAPK）之间的相互作用是真核生物信号网络的一个模型。在该担子菌中，PKA/MAPK串扰对于交配期间的信息素信号传导至关重要，这是致病性发展的先决条件。最近，我们描述了一种新的串扰机制，通过证明关键转录因子Prf 1的活性受到PKA以及MAPK磷酸化的调节。这些激酶的差异磷酸化使Prf 1能够区分各种靶基因启动子。在这个项目中，我们希望回答这个问题，Prf 1的活性是如何调节PKA以及MAPK磷酸化。此外，由于MAPK信号是需要一个特定的子集的靶基因，我们要解决的假设，第二个transciption因子与MAPK磷酸化Prf 1的行动。