Membrane-coupled mRNA trafficking in Ustilago maydis

玉米黑粉菌中膜偶联 mRNA 运输

• 批准号: 282975972
• 负责人: Professor Dr. Michael Feldbrügge
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/282975972?language=en
• 关键词: Membrane; coupled; mRNA; trafficking; Ustilago

Active transport and local translation of mRNAs are essential to precisely determine spatiotemporal protein expression. Evidence is accumulating that there is an intimate link between mRNA transport and membrane trafficking, including an intensive co-transport of mRNAs with ER or endosomes. The latter was discovered studying microtubule-dependent transport of mRNAs in infectious hyphae of the plant pathogen Ustilago maydis. In particular, transport of septin mRNA and encoded protein on shuttling endosomes is essential for efficient septin filamentation during polar growth. In this project, we will address two key aspects of membrane-coupled mRNA trafficking. Firstly, we aim to identify novel links between mRNA and membrane trafficking during polar growth of infectious hyphae. In close collaboration with partners of the research group we will perform a comparative approach in mammalian cells, Saccharomyces cerevisiae and U. maydis. Our part will be to study the role of newly identified membrane-associated RNA-binding proteins (RBPs) during hyphal growth. We will combine genetic, cell biological and biochemical approaches for functional analysis of these RBPs and uncover the link to membrane trafficking as well as target mRNAs. Secondly, we will identify and study the function of novel components of the endosomal mRNA transport machinery. To this end, we will establish and apply an in vivo proximity-dependent labelling approach in this fungal model organism using the key RBP Rrm4 as bait. In the framework of this research group, we will focus on associated RBPs needed for transport or regulatory factors that, e.g., promote the RNA-binding capacity of Rrm4. Together, the outlined experiments will yield new insight into how membrane trafficking and RNA transport are mechanistically linked. We expect to uncover new cell biological concepts conserved in evolution.

mrna的主动转运和局部翻译是精确确定时空蛋白表达的必要条件。越来越多的证据表明，mRNA运输和膜运输之间存在密切联系，包括mRNA与内质网或核内体的密集共运输。后者是在研究植物病原菌黑穗病菌（Ustilago maydis）侵染菌丝中mrna的微管依赖性转运时发现的。特别是，穿梭核内体上的septin mRNA和编码蛋白的运输对于极性生长过程中septin的高效丝化至关重要。在这个项目中，我们将解决膜偶联mRNA运输的两个关键方面。首先，我们的目标是确定在感染菌丝极性生长过程中mRNA和膜运输之间的新联系。在与研究小组的伙伴密切合作下，我们将在哺乳动物细胞，酿酒酵母和美国maydis中执行比较方法。我们的工作将是研究新发现的膜相关rna结合蛋白（rbp）在菌丝生长过程中的作用。我们将结合遗传学、细胞生物学和生化方法对这些rbp进行功能分析，并揭示其与膜运输以及靶mrna的联系。其次，我们将识别和研究内体mRNA转运机制的新成分的功能。为此，我们将以关键RBP Rrm4为诱饵，在这种真菌模型生物中建立并应用一种体内邻近依赖的标记方法。在本研究小组的框架内，我们将重点关注运输或调节因子所需的相关rbp，例如，促进Rrm4的rna结合能力。总之，概述的实验将产生新的见解如何膜运输和RNA运输是机械联系。我们期望发现在进化中保守的新的细胞生物学概念。