Replacement Phosphorimager for the Physical Biochemistry Instrument Facility at IUB

IUB 物理生化仪器设施的替换磷光成像仪

• 批准号: 0244815
• 负责人: Andrew Feig
• 金额: $ 8.27万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0244815&HistoricalAwards=false
• 关键词: Replacement; Phosphorimager; Physical; Biochemistry; Instrument

A grant has been awarded to Indiana University, Bloomington (IUB) under the direction of Dr. Andrew Feig for the acquisition of phosphorimager system that will become part of the Physical Biochemistry Instrumentation Facility. The facility houses a variety of instruments for structural, spectroscopic, thermodynamic and kinetic characterization of biochemical systems. More than 20 faculty members from at least 6 departments (Chemistry, Biology, Psychology, Medical Sciences, Physics and Optometry) use this facility although the majority of the users derive from the Chemistry and Biology Departments. The instrument acquired through this grant will be used primarily for its phosphorimager capabilities, but the model chosen also has the ability to do direct fluorescence imaging (red and green excitation) and chemiluminescence. The instrument can be upgraded in the future if blue wavelength excitation is needed. This instrument is also capable of reading microarrays. The phosphorimager will allow laboratory experiments that make use of fluorescent or chemiluminescent tags in lieu of traditional 32P labeling. Thus, excellent experiments that were removed from the undergraduate lab curriculum can be re-incorporated using this imaging system. The microarray reading capabilities of the instrument will also be used in curriculum enhancement through development of novel experiments for the undergraduate lab analyzing expression profiles during stress response. Thus, undergraduates will be exposed to state-of-the-art instrumentation in their laboratory course. The equipment will also be used by more than 50 graduate students and post-doctoral fellows. The major and minor users of the instrumentation are actively involved in several programs at IUB (MEDIC-B, IU Stars and McNair Scholars) that attract minority students interested in careers in science.

在安德鲁·费格博士的指导下，印第安纳大学布鲁明顿分校(IUB)获得了一笔赠款，用于购买将成为物理化学仪器设施一部分的磷成像系统。该设施配备了各种仪器，用于生化系统的结构、光谱、热力学和动力学表征。来自至少6个系(化学、生物、心理学、医学、物理和验光)的20多名教职员工使用这一设施，尽管大多数用户来自化学和生物系。通过这笔赠款获得的仪器将主要用于其磷成像能力，但所选的型号还具有直接荧光成像(红色和绿色激发)和化学发光的能力。如果需要蓝光激发，将来可以对仪器进行升级。该仪器还能够读取微阵列。磷成像仪将允许实验室实验使用荧光或化学发光标签来取代传统的32P标记。因此，从本科实验室课程中删除的优秀实验可以使用该成像系统重新纳入。该仪器的微阵列读取能力也将用于课程改进，方法是为本科生实验室开发新的实验，分析应激反应中的表达谱。因此，本科生将在他们的实验室课程中接触到最先进的仪器。该设备还将供50多名研究生和博士后使用。仪器的主要用户和次要用户积极参与了IUB的几个项目(MEDEC-B、IU Stars和McNair Scholars)，这些项目吸引了对科学职业感兴趣的少数族裔学生。