A phosphorimager for imaging radioactively-labeled nucleic acid samples

用于对放射性标记的核酸样品进行成像的磷光成像仪

• 批准号: 374640-2009
• 负责人: Sen, Dipankar
• 金额: $ 4.17万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=411976
• 关键词: phosphorimager; imaging; radioactively; labeled; nucleic

We request a new phosphorimager. This piece of equipment will functionally substitute for an existing Typhoon 9410 phosporimager that is both nearing the end of its operational life and is heavily used by diverse constituencies at Simon Fraser University. The new phosphorimager will enable the routine nucleic acid radioactive imaging procedures that are essential to both the Sen and Unrau laboratories. Both laboratories make extensive use of 32P radiolabel to study nucleic acids structure and function, and both groups critically depend on their access to the aging Typhoon phosphorimager. Last year our laboratories were the primary users of the 32P isotope at Simon Fraser University, purchasing 50.2% of all source vials and utilizing 60.6% of all activity (3644 MBq). The equipment we request is essential to ensure the ongoing timeliness and vitality of the research carried out by our two laboratories.

我们需要一台新的磷光成像仪。这台设备将在功能上取代现有的台风9410磷成像仪，该成像仪的使用寿命即将结束，西蒙·弗雷泽大学的不同支持者大量使用该成像仪。新的磷成像仪将使常规的核酸放射性成像程序成为可能，而这对森实验室和银河实验室都是必不可少的。这两个实验室都广泛使用32P放射性标记来研究核酸的结构和功能，而且两个小组都严重依赖于他们能否接触到老化的台风磷成像仪。去年，我们的实验室是西蒙弗雷泽大学32P同位素的主要用户，购买了所有来源小瓶的50.2%，利用了所有活动的60.6%(3644MBq)。我们要求的设备对于确保我们两个实验室进行的研究的持续及时性和活力至关重要。