Position and Orientation Specific Immobilization of Antibodies via Nanofabrication and Polyvalent Interactions

通过纳米加工和多价相互作用对抗体进行位置和方向特异性固定

• 批准号: 0244830
• 负责人: Gang-Yu Liu
• 金额: $ 34.5万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0244830&HistoricalAwards=false
• 关键词: Position; Orientation; Specific; Immobilization; Antibodies

Professor Gang-Yu Liu of the University of California Davis is supported by the Analytical and Surface Chemistry program to fabricate nanostructures that immobilize antibodies with designated positions while maintaining their bioreactivity. Scanning probe tips are used to secure antigens followed by divalent binding of antibodies. The structures are probed by scanning probe microscopy (SPM). Can the assemblies trigger cell responses which mimic natural immune processes? These studies have the potential to open new avenues of study in bioanalytical and bioengineering areas. The project also relates to the goals of miniaturizing electronics such as biosensors to molecular-size scale. Students receive interdisciplinary training in electrochemistry and nanofabrication skills and benefit from close collaboration with a synthetic chemist and an immunologist.

加州大学戴维斯分校的刘刚宇教授得到了分析和表面化学计划的支持，该计划旨在制造纳米结构，在保持抗体生物反应性的同时将抗体固定在指定位置。扫描探针尖被用来保护抗原，然后是抗体的二价结合。用扫描探针显微镜(SPM)对其结构进行了分析。这些集合体能否触发模拟自然免疫过程的细胞反应？这些研究有可能在生物分析和生物工程领域开辟新的研究途径。该项目还涉及到将生物传感器等电子设备微型化到分子大小的目标。学生接受电化学和纳米制造技能的跨学科培训，并从与合成化学家和免疫学家的密切合作中受益。