Molecular basis of primordial germ cell formation from multipotent stem cells in the polychaete Platynereis dumerilii, an ancestral lophotrochozoan.

多毛类 Platynereis dumerilii（一种祖先冠轮动物）多能干细胞形成原始生殖细胞的分子基础。

• 批准号: 161759413
• 负责人: Dr. Nicole Rebscher
• 金额: --
• 依托单位: Fachbereich 17: Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161759413?language=en
• 关键词: Molecular; basis; primordial; germ; cell

Basal metazoans such as cnidarians and platyhelminthes exhibit stem cells, which give rise to both somatic descendants and primordial germ cells. Recently we could show that similar multipotent stem cells exist in the growth zone of the polychaete Platynereis du-merilii. In order to elucidate the molecular mechanism underlying the formation of germ cells from stem cells in this lophotrochozoan, this project follows three approaches: (1) The function of the germ cell-related genes vasa and nanos will be elucidated by RNAi. (2) Injections of capped mRNA from EGFP:3’UTR constructs into fertilized eggs will pro-vide information on the role of the 3’untranslated region of vasa and nanos in the stabiliza-tion of the corresponding transcripts. Furthermore, this approach will not only allow visual-izing and manipulating of the primordial germ cells of Platynereis for the first time in vivo, but also unraveling regulatory elements and putative micro-RNA binding sites in the 3’UTRs. (3) inhibitor experiments will identify the signaling cascades (FGFR, Notch, Wnt, MAPK) involved in PGC specification. Besides unraveling the molecular mechanism of germ cell formation in Platynereis, this project will contribute to a better understanding of the evolutionary relationship between germ cells and stem cells in Metazoa.

基础后生动物如刺胞动物和扁形动物表现出干细胞，它们产生体细胞后代和原始生殖细胞。最近，我们可以表明，类似的多能干细胞存在于多毛类Platynereis du-merilii的生长区。本研究从以下三个方面探讨了该线虫从干细胞分化为生殖细胞的分子机制：（1）利用RNAi技术研究生殖细胞相关基因vasa和nanos的功能。(2)将EGFP：3 'UTR结构的加帽mRNA注射到受精卵中将提供vasa和nanos的3'非翻译区在相应转录物稳定中的作用的信息。此外，这种方法不仅允许可视化和操纵的原始生殖细胞的Platynereis的第一次在体内，而且解开调控元件和推定的micro-RNA结合位点的3 'UTR。(3)抑制剂实验将鉴定参与PGC特化的信号传导级联（FGFR、Notch、Wnt、MAPK）。本研究除了揭示扁水母类生殖细胞形成的分子机制外，还将有助于更好地理解后生动物生殖细胞和干细胞之间的进化关系。