A Capillary DNA Sequencer and Denaturing HPLC for Molecular Genetics, Ecogenomics, and Experimental Bioinformatics

在托马斯道林博士的指导下，亚利桑那州立大学获得了一笔赠款，用于购买一台48毛细管DNA测序仪/遗传分析仪，以及一台HPLC基因分型和DNA片段纯化系统。 这种仪器是必不可少的有效完成的项目正在亚利桑那州立大学进行的多样性，包括分子，进化，生态，基因组和生物信息学研究。购置这种设备是必要的，以处理现有和新的教师不断增加的需求（样本数量），并将允许调查人员解决目前无法接近现有系统的问题。将利用仪器的研究领域包括：（1）分子遗传学和光合作用的进化，（2）分子系统学和生物多样性的进化（例如，细菌，真菌，植物，动物），（3）濒危物种的遗传学和管理，（4）渐渗杂交和进化基因组学，（5）种群生物学。该设备将成为该大学DNA测序设施的一部分。该设施为多个教育项目提供支持，如NSF REU和UMEB项目，这些项目向不同背景的学生介绍科学职业。训练这些学生的核心是接触最新的技术，使我们能够获得以前无法获得的答案。因此，增加这些设备将使我们能够更好地为在我们实验室工作的本科生和毕业生提供服务，让他们接触到最先进的技术和技巧。

A grant has been awarded to Arizona State University under the direction of Dr. Thomas Dowling for the acquisition of a 48-capillary DNA sequencer/genetic analyzer, and an HPLC genotyping and DNA fragment purification system. This instrumentation is essential for efficient completion of the diversity of projects being conducted at ASU, including molecular, evolutionary, ecological, genomic, and bioinformatic studies. Acquisition of this equipment is necessary to handle both increasing demands (number of samples) by existing and new faculty, and will permit investigators to address questions not currently approachable with the existing systems. Research areas that will utilize the instrumentation include: (1) molecular genetics and the evolution of photosynthesis, (2) molecular systematics and evolution of a diversity of organisms (e.g., bacteria, fungi, plants, animals), (3) genetics and management of endangered species, (4) introgressive hybridization and evolutionary genomics, and (5) population biology. The equipment will become part of the DNA sequencing facility at the university. This facility provides support to several educational programs, such as the NSF REU and UMEB programs, which introduce students of diverse backgrounds to careers in science. Central to training these students is exposure to the newest techniques that allow us to obtain previously unobtainable answers. Therefore, addition of this equipment will allow us to better serve the undergraduates and graduates working in our laboratories by exposing them to state of the art technology and techniques.