High-Throughput DNA Sequencer

 描述(由申请人提供) UCI基因组学高吞吐量设施(GHTF)请求共享仪器授权支持HiSeq 3000下一代DNA测序仪，以补充和取代当前的HiSeq 2500。HiSeq 3000是一款最先进的DNA测序仪，用于生物医学研究、基因组学和基因表达研究。该测序仪在3.5天内产生750G碱基的序列，错误率约为0.1%。GHTF是UCI校园和医疗中心唯一的基因组研究核心设施。它的使命是向研究界提供超出个人研究人员资源的新兴和当前技术。除了UCI社区，它还为缺乏测序设施的地区公司和大学提供服务。它已经存在了16年，并通过不断向研究界提供新兴技术方面的高质量援助，通过几次技术变革而蓬勃发展。除了Illumina DNA测序，GHTF还提供PacBioRS远程单分子DNA测序、Affymetrix微阵列、NanoString数字RNA计数和Fluidigm C1单细胞分离。随着应用的多样化，灯盏花DNA测序正在迅速发展，目前是GHTF提供的主要研究支持。HiSeq 3000每次运行产生的序列是目前GHTF HiSeq 2500 v3 DNA测序仪的两倍多，所需时间约为三分之一--成本大致相同。因此，获得测序仪对GHTF至关重要。目前，GHTF在样品制备和DNA和RNA序列分析的所有方面为大约50名UCI调查人员提供支持，包括芯片、甲基、RNA和DNA序列分析。该申请描述了27名生物医学研究人员的项目，包括对疾病和正常组织的调查：黑色素瘤、乳腺癌和结肠癌、卢格里克病、心肌病、酒精中毒对肝功能的影响、药物成瘾、正常组织形态形成和干细胞功能、记忆、皮肤再生、线粒体功能、干细胞分化、DNA修饰和神经元可塑性、RNA剪接和聚腺苷酸化。最近，GHTF获得了对单细胞进行DNA和RNA测序的能力，并正在研究这项技术，并正在与研究人员合作，开发在单个细胞水平上理解免疫、皮肤和神经系统的新程序。GHTF是整合网络细胞特征神经库(LINCS)计划的RNA测序中心，该计划正在为肌萎缩侧索硬化症/卢·格里克病开发完整的细胞图谱。GHTF提供商业供应商的技术研讨会和研讨会，为校园研究人员提供生物信息学研讨会，生物信息学服务支持，以及校园研讨会。HiSeq 3000是支持这些GHTF功能的关键仪器。

 DESCRIPTION (provided by applicant) The UCI Genomics High-Throughput Facility (GHTF) requests Shared Instrument Grant Support for a HiSeq 3000 next generation DNA sequencer to supplement and replace the current HiSeq 2500. The HiSeq 3000 is a state-of-the-art, DNA sequencer for biomedical research genomics and gene expression studies. This sequencer generates 750 Gbases of sequence in 3.5 days with an error rate of ~0.1%. The GHTF is the sole genomics research Core Facility for the UCI campus and Medical Center. Its mission is to make available to the research community emergent and current technologies that exceed the resources of the individual investigator. In addition to the UCI community, it offers services to regional companies and colleges that lack sequencing facilities. It has existed for sixteen years and flourished through several technological transformations by consistently offering high quality assistance in emerging technologies to the research community. In addition to Illumina DNA sequencing the GHTF offers PacBioRS long-range, single-molecule DNA sequencing, Affymetrix microarrays, Nanostring digital RNA counting, and Fluidigm C1 single cell fractionation. Illumina DNA sequencing with diversifying applications is growing rapidly and is currently the major research support offered by the GHTF. The HiSeq 3000 is capable of generating more than twice as much sequence per run as the current GHTF HiSeq 2500 v3 DNA sequencer, in about one-third of the time-at roughly the same cost. Acquisition of the sequencer is therefore essential to the GHTF. Currently the GHTF supports about fifty UCI investigators in all aspects of sample preparation and DNA and RNA sequence analysis including ChIP-, methyl-, RNA-, and DNA-seq. This application describes projects of twenty-seven biomedical researchers including investigations into diseased and normal tissue: melanoma, breast and colon cancer, Lou Gehrig's Disease, cardiomyopathy, alcoholism effect on liver function, drug addiction, and normal tissue morphogenesis and stem cell function, memory, skin regeneration, mitochondrial function, stem cell differentiation, DNA modification and neuronal plasticity, RNA splicing and polyadenylation. Most recently, the GHTF acquired the capability to perform DNA and RNA sequencing of single cells and is pursuing this technology and is working with investigators to develop new programs in understanding immune, skin and nervous systems at the level of the individual cell. The GHTF is the RNA sequencing center for the Neuro Library of Integrated Network-based Cellular Signatures (LINCS) program which is developing a complete cellular profile for Amyotrophic lateral sclerosis/Lou Gehrig's Disease. The GHTF provides technical seminars and workshops by commercial venders, workshops in bioinformatics for campus researchers, bioinformatics service support, and campus symposia. The HiSeq 3000 is a critical instrument in support of these GHTF functions.