Next Generation DNA Sequencer

 描述（由申请人提供）： 自从我们获得第一台下一代仪器以来，冷泉港实验室的科学家们一直在积极地将下一代测序纳入NIH支持的广泛研究项目中。我们目前的仪器主要是Illumina HiSeq 2000。这些仪器于2010年推出，并已在市场上被Illumina HiSeq 2500取代。我们目前在CSHL没有HiSeq 2500。与HiSeq 2000相比，HiSeq 2500提供了更高的输出、更长的质量读取长度和更低的数据单位成本。此外，Illumina宣布的新化学将提供比HiSeq 2500更高的成本效率和更快的运行时间。这种新的化学物质将仅适用于较新的HiSeq 2500仪器，而不是我们的旧型号。我们正在申请资金购买Illumina HiSeq 2500，以便我们能够继续为NIH资助的CSHL社区研究人员提供最先进，具有成本效益的下一代测序。

 DESCRIPTION (provided by applicant): The scientists at Cold Spring Harbor Laboratory have been actively incorporating next generations sequencing into a broad range of NIH supported research projects since we obtained our first next generation instrument. Our current instruments are primarily Illumina HiSeq 2000s. These instruments were introduced in 2010 and have been replaced on the market by the Illumina HiSeq 2500. We currently have no HiSeq 2500s at CSHL. The HiSeq 2500 provides higher output, longer quality read length and a lower cost per base for data than does the HiSeq 2000. In addition, new chemistry that has been announced by Illumina will provide even greater cost efficiency and faster run times than are currently available on the HiSeq 2500. This new chemistry will only be available for the newer HiSeq 2500 instruments, not our older models. We are requesting funds to purchase an Illumina HiSeq 2500 so that we can continue providing state of the art, cost effective next generation sequencing to the CSHL community of NIH funded researchers.